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Bin1 (NM_001083334) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG207643
Bin1 (tGFP-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bin1
Synonyms ALP-1; Amphl; BRAMP-2; SH3P9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207643 representing NM_001083334
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGATGGGGAGCAAGGGGGTGACGGCGGGGAAGATCGCCAGCAACGTACAGAAGAAGCTGACCC
GAGCGCAGGAGAAGGTCCTGCAGAAACTGGGGAAGGCGGACGAGACGAAGGACGAGCAGTTTGAGCAGTG
TGTCCAGAACTTCAATAAGCAGCTGACAGAGGGTACCCGGCTGCAGAAGGATCTTCGGACCTATCTGGCT
TCTGTTAAAGCGATGCACGAAGCCTCCAAGAAGCTGAGTGAGTGTCTTCAGGAGGTGTATGAGCCCGAGT
GGCCTGGCAGGGATGAAGCAAACAAGATTGCAGAGAACAATGACCTACTCTGGATGGACTACCACCAGAA
GCTGGTGGACCAGGCTCTGCTGACCATGGACACCTACCTAGGCCAGTTCCCTGATATCAAGTCGCGCATT
GCCAAGCGGGGGCGGAAGCTGGTGGACTATGACAGTGCCCGGCACCACTATGAGTCTCTTCAAACCGCCA
AAAAGAAGGATGAAGCCAAAATTGCCAAGGCAGAAGAGGAGCTCATCAAAGCCCAGAAGGTGTTCGAGGA
GATGAACGTGGATCTGCAGGAGGAGCTGCCATCCCTGTGGAACAGCCGTGTAGGTTTCTATGTCAACACG
TTCCAGAGCATCGCGGGTCTGGAGGAAAACTTCCATAAAGAGATGAGTAAGCTCAATCAGAACCTCAATG
ATGTCCTGGTCAGCCTAGAGAAGCAGCACGGGAGCAACACCTTCACAGTCAAGGCCCAACCCAGTGACAA
TGCCCCTGAGAAAGGGAACAAGAGCCCGTCACCTCCTCCAGATGGCTCCCCTGCTGCTACCCCTGAGATC
AGAGTGAACCATGAGCCAGAGCCGGCCAGTGGGGCCTCACCCGGGGCTACCATCCCCAAGTCCCCATCTC
AGCTCCGGAAAGGCCCACCTGTCCCTCCGCCTCCCAAACACACCCCATCCAAGGAGATGAAGCAGGAGCA
GATTCTCAGCCTTTTTGATGACGCATTTGTCCCTGAGATCAGCGTGACCACCCCCTCCCAGCCAGCAGAG
GCCTCCGAGGTGGTGGGTGGAGCCCAGGAGCCAGGGGAGACAGCAGCCAGTGAAGCAACCTCCAGCTCTC
TTCCGGCTGTGGTGGTGGAGACCTTCTCCGCAACTGTGAATGGGGCGGTGGAGGGCAGCGCTGGGACTGG
ACGCTTGGACCTGCCCCCGGGATTCATGTTCAAGGTTCAAGCCCAGCATGATTACACGGCCACTGACACT
GATGAGCTGCAACTCAAAGCTGGCGATGTGGTGTTGGTGATTCCTTTCCAGAACCCAGAGGAGCAGGATG
AAGGCTGGCTCATGGGTGTGAAGGAGAGCGACTGGAATCAGCACAAGGAACTGGAGAAATGCCGCGGCGT
CTTCCCGGAGAATTTTACAGAGCGGGTGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207643 representing NM_001083334
Red=Cloning site Green=Tags(s)

MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLA
SVKAMHEASKKLSECLQEVYEPEWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRI
AKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNT
FQSIAGLEENFHKEMSKLNQNLNDVLVSLEKQHGSNTFTVKAQPSDNAPEKGNKSPSPPPDGSPAATPEI
RVNHEPEPASGASPGATIPKSPSQLRKGPPVPPPPKHTPSKEMKQEQILSLFDDAFVPEISVTTPSQPAE
ASEVVGGAQEPGETAASEATSSSLPAVVVETFSATVNGAVEGSAGTGRLDLPPGFMFKVQAQHDYTATDT
DELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001083334
ORF Size 1431 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001083334.1, NP_001076803.1
RefSeq Size 2135 bp
RefSeq ORF 1434 bp
Locus ID 30948
Cytogenetics 18 18.01 cM
Summary Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:12183633). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:12668730, PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001083334" in other vectors (4)
SKU Description Size Price
MC202551 Bin1 (untagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2, (10ug) 10 ug
$457.00
MR207643 Bin1 (Myc-DDK-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 10 ug
$289.00 $457.00
MR207643L3 Lenti ORF clone of Bin1 (Myc-DDK-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 10 ug
$757.00
MR207643L4 Lenti ORF clone of Bin1 (mGFP-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 10 ug
$757.00

Plasmid Map for MG207643

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG207643
Circular map for MG207643

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