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Shc1 (NM_011368) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207498
Shc1 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 2
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Shc1
Synonyms p66; p66shc; Shc; ShcA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207498 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCGGGTAGAAGGGGGCCAGCTGGGGGGCGAGGAGT
GGACCAGACACGGGAGCTTTGTCAATAAGCCCACACGAGGCTGGCTGCATCCCAACGACAAAGTCATGGG
ACCTGGGGTTTCCTACTTGGTTCGGTACATGGGCTGTGTGGAGGTCTTACAGTCAATGCGAGCCCTTGAC
TTCAATACCCGGACTCAGGTCACCAGGGAGGCCATCAGTTTGGTGTGTGAAGCTGTGCCTGGTGCCAAAG
GGGCGACAAGGAGGAGAAAGCCTTGTAGCCGCCCACTCAGCTCCATCCTGGGGAGGAGTAACCTGAAGTT
TGCTGGAATGCCAATCACTCTCACTGTGTCTACCAGCAGCCTTAACCTCATGGCAGCCGACTGCAAACAG
ATCATTGCCAACCATCACATGCAATCTATCTCTTTCGCGTCCGGTGGGGATCCGGACACAGCTGAGTATG
TTGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCATATCCTGGAGTGTCCTGAAGGGCTTGC
TCAGGATGTCATCAGCACCATCGGGCAGGCCTTTGAGTTGCGCTTCAAACAGTATCTCAGGAATCCACCG
AAGCTGGTCACCCCCCATGACAGGATGGCTGGCTTTGATGGCTCAGCTTGGGATGAGGAGGAAGAAGAGC
CCCCTGACCATCAGTACTACAATGACTTTCCAGGGAAGGAACCCCCTCTTGGTGGGGTGGTAGATATGAG
GCTTCGGGAAGGGGCTGCTCGACCCACTCTGCCTAGTGCCCAGATGTCCAGCCACTTGGGAGCTACACTG
CCTATAGGGCAGCATGCTGCAGGAGACCATGAAGTCCGTAAACAGATGTTGCCTCCGCCGCCTTGCCCAG
GCAGAGAACTCTTCGATGACCCCTCCTATGTCAACATCCAGAATCTAGACAAGGCCCGGCAGGCTGGGGG
TGGGGCTGGGCCCCCAAATCCTTCTCTTAATGGCAGTGCACCCCGAGACCTTTTTGACATGAAGCCCTTT
GAAGATGCACTTCGGGTGCCACCCCCACCGCAGTCCATGTCCATGGCTGAGCAGCTGCAAGGGGAGCCCT
GGTTCCACGGGAAGCTGAGCCGGAGGGAGGCCGAGGCGCTGCTGCAGCTCAATGGTGACTTCTTGGTGCG
AGAGAGCACGACCACGCCTGGCCAGTATGTGCTCACTGGCCTGCAGAGTGGGCAGCCCAAGCACTTGCTG
CTGGTGGACCCTGAAGGTGTGGTTCGGACAAAGGATCACCGCTTTGAGAGTGTCAGTCACCTGATCAGCT
ACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGCCTACAGCAACCCGTGGATCG
GAAAGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207498 protein sequence
Red=Cloning site Green=Tags(s)

MNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSMRALD
FNTRTQVTREAISLVCEAVPGAKGATRRRKPCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQ
IIANHHMQSISFASGGDPDTAEYVAYVAKDPVNQRACHILECPEGLAQDVISTIGQAFELRFKQYLRNPP
KLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAARPTLPSAQMSSHLGATL
PIGQHAAGDHEVRKQMLPPPPCPGRELFDDPSYVNIQNLDKARQAGGGAGPPNPSLNGSAPRDLFDMKPF
EDALRVPPPPQSMSMAEQLQGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLL
LVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVDRKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011368
ORF Size 1407 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011368.5, NP_035498.2
RefSeq Size 3171 bp
RefSeq ORF 1410 bp
Locus ID 20416
UniProt ID P98083
Cytogenetics 3 39.11 cM
MW 51.4 kDa
Summary Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis (By similarity). Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.UniProtKB/Swiss-Prot Function
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"NM_011368" in other vectors (3)
SKU Description Size Price
MC216286 Shc1 (untagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 2, (10ug) 10 ug
$503.00
MR207498L3 Lenti ORF clone of Shc1 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 2 10 ug
$757.00
MR207498L4 Lenti ORF clone of Shc1 (mGFP-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 2 10 ug
$757.00

Plasmid Map for MR207498

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR207498
Circular map for MR207498

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