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Dmap1 (NM_023178) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207480
Dmap1 (Myc-DDK-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Dmap1 or NM_023178"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dmap1
Synonyms 1500016M21Rik; Dmtap1; DNMAP1; DNMTAP1; mKIAA1425
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207480 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACGGGCGCAGATGTACGAGACATTCTAGAACTCGGGGGTCCAGAGGGAGATGCCGCCTCTGGGA
CCATCAGCAAAAAGGATATTATCAACCCGGACAAGAAAAAGTCCAAGAAGTCCTCAGAGACGCTGACCTT
CAAGAGGCCTGAGGGCATGCATCGGGAGGTCTATGCTTTGCTTTACTCTGACAAAAAGGATGCACCCCCA
CTGCTGCCCAGTGACACTGGTCAGGGGTATCGGACAGTGAAGGCGAAACTGGGGTCCAAGAAGGTTCGCC
CTTGGAAATGGATGCCTTTTACTAACCCAGCTCGAAAGGACGGCGCTATGTTTTTCCACTGGCGACGAGC
GGCGGAGGAGGGCAAGGACTACCCTTTTGCCAGGTTCAATAAGACGGTGCAGGTGCCCGTGTACTCAGAG
CAGGAGTACCAACTCTACCTTCATGATGACGCATGGACTAAGGCAGAGACTGACCACCTATTTGACCTCA
GCCGCCGATTTGATCTGCGCTTCGTAGTTATTCACGATCGGTATGACCACCAGCAGTTCAAGAAGCGTTC
TGTGGAGGACCTGAAAGAGAGGTACTACCACATTTGTGCCAAGCTTGCCAACGTGAGGGCTGTGCCAGGC
ACAGATCTCAAGATACCAGTGTTTGATGCTGGGCATGAGAGACGGCGGAAGGAACAGCTAGAGCGGCTTT
ACAACCGAACCCCAGAGCAGGTGGCAGAGGAGGAGTACCTCCTACAGGAGCTGCGTAAGATTGAGGCCCG
GAAAAAAGAGCGGGAGAAGCGCAGCCAAGACCTGCAGAAGCTGATTACAGCAGCAGACACCACTGCAGAG
CAGCGGCGCACGGAACGCAAGGCTCCCAAGAAGAAGCTACCCCAAAAGAAGGAGGCTGAGAAGCCGGCTG
TCCCTGAGACTGCAGGCATCAAGTTTCCAGATTTTAAGTCGGCAGGTGTCACGCTACGGAGCCAGCGGAT
GAAGCTACCCAGCTCTGTGGGTCAGAAGAAGATCAAGGCGCTGGAACAGATGCTGCTGGAACTTGGTGTG
GAGCTGAGCCCTACCCCCACAGAGGAGCTGGTGCATATGTTCAATGAGTTGCGGAGCGACCTGGTGTTAC
TCTACGAGCTCAAGCAGGCCTGTGCCAACTGTGAATATGAGCTACAGATGCTGCGGCACCGGCACGAGGC
CCTGGCTCGGGCAGGAGTGCTGGGGGCCCCTGCCGCAGCAGCAGTGGGACCAACCCCGGCTTCTGCTGAG
CCAACAGTGTCTGAATCTGGACTTGGTCTGGACCCCACCAAGGATACCATCATTGATGTCGTGGGTGCAC
CCCTCACACCCAATTCGCGGAAACGACGGGAATCAGCCTCCAGCTCATCTTCTGTGAAGAAAGCCAAGAA
ACCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207480 protein sequence
Red=Cloning site Green=Tags(s)

MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYALLYSDKKDAPP
LLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEEGKDYPFARFNKTVQVPVYSE
QEYQLYLHDDAWTKAETDHLFDLSRRFDLRFVVIHDRYDHQQFKKRSVEDLKERYYHICAKLANVRAVPG
TDLKIPVFDAGHERRRKEQLERLYNRTPEQVAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAE
QRRTERKAPKKKLPQKKEAEKPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGV
ELSPTPTEELVHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGAPAAAAVGPTPASAE
PTVSESGLGLDPTKDTIIDVVGAPLTPNSRKRRESASSSSSVKKAKKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023178
ORF Size 1404 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023178.2, NP_075667.1
RefSeq Size 1543 bp
RefSeq ORF 1407 bp
Locus ID 66233
UniProt ID Q9JI44
Cytogenetics 4 D2.1
MW 53.1 kDa
Summary Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023178" in other vectors (4)
SKU Description Size Price
MC216224 Dmap1 (untagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1), (10ug) 10 ug
$503.00
MG207480 Dmap1 (tGFP-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) 10 ug
$657.00
MR207480L3 Lenti ORF clone of Dmap1 (Myc-DDK-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) 10 ug
$757.00
MR207480L4 Lenti ORF clone of Dmap1 (mGFP-tagged) - Mouse DNA methyltransferase 1-associated protein 1 (Dmap1) 10 ug
$757.00

Plasmid Map for MR207480

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR207480
Circular map for MR207480

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