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Rgma (BC023870) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206975
Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rgma
Synonyms C230063O06, MGC38550, MGC69915
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206975 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTATGGGGAGAGGGGCAGGACGTTCAGCCCTGGGATTGTGGCCGACCCTCGCCTTCCTTCTCTGCA
GCTTCCCCGCAGCCATCTCCCCCTGCAAAATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCATC
AGGCAGCCACGCCCCTGCCTCCGACGACGTGCCGGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGC
ACTCGGCGGACAGCCCGCACCTGCCGGGGCGACCTGGCTTACCACTCGGCTGTCCATGGCATAGAGGACC
TTATGAGCCAGCACAACTGCTCCAAGGACGGCCCCACCTCACAGCCGCGAGTGCGCACGCTCCCGCCAGC
TGGGGACAGCCAGGAGCGCTCGGATAGCCCCGAGATCTGCCACTATGAGAAGAGTTTCCACAAGCACTCA
GCTGCCCCCAACTACACTCACTGCGGCCTCTTTGGGGACCCACACCTCAGGACTTTCACAGACCACTTCC
AGACATGCAAGGTGCAAGGCGCCTGGCCTCTCATCGACAATAATTACCTGAACGTGCAAGTCACCAATAC
ACCTGTGCTGCCGGGCTCCGCAGCTACCGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAAGAG
TGTGTGGACCAGAAAGTCTACCAAGCTGAAATGGACGAACTTCCGTCTGCCTTTGCGGACGGCTCCAAAA
ATGGGGGAGATAAACACGGGGCCAACAGCCTGAAGATCACAGAGAAGGTGTCGGGCCAGCACGTGGAGAT
CCAGGCCAAGTACATCGGCACCACCATCGTGGTGCGACAGGTGGGCCGCTACCTGACCTTTGCCGTCCGG
ATGCCCGAGGAGGTAGTCAACGCCGTGGAGGACCGTGACAGCCAAGGCCTCTACCTCTGCCTGCGGGGCT
GCCCGCTCAACCAGCAGATCGACTTCCAGGCTTTCCGTGCCAACGCTGAAAGCCCTCGCAGGCCAGCAGC
CGCCAGTCCCTCTCCCGTGGTCCCCGAGACATTCCCCTATGAGACAGCCGTGGCCAAGTGCAAAGAGAAG
CTGCCCGTAGAAGACCTGTACTACCAGGCCTGTGTCTTCGACCTCCTCACGACTGGTGATGTGAACTTCA
CGCTGGCTGCCTACTATGCTTTGGAGGATGGCAAGATGCTCCACTCCAACAAGGACAAGCTGCATCTGTT
TGAAAGGACTCGGGAGCTGCCAGGAGCCGTGGCCGCCGCCGCCGCTGCCACCACATTCCCCTTGGCCCCC
CAGATTCTCCTTGGCACCATCCCACTTCTGGTCCTCCTGCCTGTGTTGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206975 protein sequence
Red=Cloning site Green=Tags(s)

MGMGRGAGRSALGLWPTLAFLLCSFPAAISPCKILKCNSEFWSATSSGSHAPASDDVPEFCAALRTYALC
TRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRVRTLPPAGDSQERSDSPEICHYEKSFHKHS
AAPNYTHCGLFGDPHLRTFTDHFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQE
CVDQKVYQAEMDELPSAFADGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVR
MPEEVVNAVEDRDSQGLYLCLRGCPLNQQIDFQAFRANAESPRRPAAASPSPVVPETFPYETAVAKCKEK
LPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDGKMLHSNKDKLHLFERTRELPGAVAAAAAATTFPLAP
QILLGTIPLLVLLPVLW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023870
ORF Size 1311 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023870, AAH23870
RefSeq Size 3384 bp
RefSeq ORF 1313 bp
Locus ID 244058
Cytogenetics 7 D1
MW 47.8 kDa
Summary Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.UniProtKB/Swiss-Prot Function
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Documents
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"BC023870" in other vectors (4)
SKU Description Size Price
MC218652 Rgma (untagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958), (10ug) 10 ug
$503.00
MG206975 Rgma (tGFP-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) 10 ug
$489.00 $657.00
MR206975L3 Lenti ORF clone of Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) 10 ug
$757.00
MR206975L4 Lenti ORF clone of Rgma (mGFP-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) 10 ug
$757.00

Plasmid Map for MR206975

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206975
Circular map for MR206975

file-alt Citations

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling
Robinson, Griffiths, van de Haar et al
Cell (2021) 184 (8), 2103-2120.e31 DOI: 10.1016/j.cell.2021.02.045
Cited by 43
AI Summary: OriGene contributed to this study by providing the pCMV-mycDDK-RGMA plasmid (Cat# MR206975), which was used to express RGMA and investigate its role in NEO1 signaling and complex formation.
Snippet: TGTAATCGATATC This paper N/A ------------------------- Recombinant DNA ------------------------- Plasmid: pCX-GFP Alain Chédotal (Zelina et al., 2014) N/A Plasmid: pCMV-mycDDK-RGMA Origene Cat# MR206975 Plasmid: pCAG:mNtn1-/3xGS/mCherry Custom made at Vector Builder (Brignani et al., 2020) Vector ID: VB190710-1048nbz Plasmid: pSuper-shNeogenin (van Erp et al., 2015;Plasmid: pCMV-mycDDK-RGMA Origene
Showing 1-1 of 1 papers.
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