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Sptlc3 (NM_175467) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206971
Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sptlc3
Synonyms C130053K05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206971 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAATCTGAACGACAGTGCTGTTACAAATGGGACTCTACACAATCCTAAGACCCAGCAAGGAAAAA
GGCAAAGCACTGGTTGTGTGAAAAATGGAATCTCAAAGGAAGCCCAGCAAAATAGGAAGGCATATGCTGA
GGATAAGCCAGTCTTTGAGCCCTACCAGGAAGCACCACTTTATGTCTACGTTTTAACTTACATGGGATAT
GGAATTGGAATCCTATTCGGCTATCTCAGAGACTTTATGAGAAACTGGGGAATTGAAAAATGCAATGCAG
CTGTAGAGCGAGAAGAACAAAAAGACTTTGTGCCGCTGTATCAGGACTTTGAGAACTTTTATAAGAGGAA
CCTTTACATGAGAATCAGAGACAGCTGGAGTCACACGGTCTGCAGTGCCCCAGAGCCTTACATGAATGTC
ATGGAGAAAGTGACTGATGACTATAACTGGACATTCAGGCATACTGGAAAAGTTATAGAAAATATCATCA
ACATGGCCTCCTACAACTACCTTGGGCTTGCAGGGAAATATGATGATTCGATGGTGAGAGTTAAGGATAC
TTTAGAAAAGTATGGTGTAGGTGTGGCCAGCACCAGAAATGAAATGGGTACCTTGGATATACACAAGGAG
TTGGAGGATCTCATGGCTGAGTTCTTGAATGTAGAAGCAGTGATGTCTTTTGGAATGGGATTCGCAACCA
ACGCAATGAATATCCCTGTATTTGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTTAACCACACTTC
AGTCATCCTAGGATCTCGACTCTCAGGTGCAGTCATAAGACCCTTCAAACACAATAATGCAGAAAACCTA
GAGAAGCTCCTGAGAGAAGCTATCATCCGTGGCCAGCCTGGCACAGGGAGAGCTTGGAAAAAGATTCTCA
TCGTGGTAGAGGGCGTCTACAGCATGGAAGGTTCCATTGTGAACCTTGCCCAGATAGTGGCTCTAAAGAA
GAAATACAAGGCTTACCTGTACATTGATGAAGCTCACAGTATTGGCTGTACAGGCCCCACGGGACGAGGC
GTCAGAGAGTTATTTGGACTGGACCCTGAAGATATTGACGTATACATGGGTACATTCACCAAGAGCTTCT
CGGGCTCAGGAGGATATATAGGCGGGAAAAAGGAAATTGTGGATTATTTACGGATGCAGTCACATAGCAC
TACTTATGCTACATCCATGAGTCCCGTAGTAGCAGCACAACTCATCAGATCATTGAAGATAACTATGGAT
ATGAGGGGAACATTGGAGGTATGGAGAGAATACAGCAACTTAAGGAAAACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206971 protein sequence
Red=Cloning site Green=Tags(s)

MANLNDSAVTNGTLHNPKTQQGKRQSTGCVKNGISKEAQQNRKAYAEDKPVFEPYQEAPLYVYVLTYMGY
GIGILFGYLRDFMRNWGIEKCNAAVEREEQKDFVPLYQDFENFYKRNLYMRIRDSWSHTVCSAPEPYMNV
MEKVTDDYNWTFRHTGKVIENIINMASYNYLGLAGKYDDSMVRVKDTLEKYGVGVASTRNEMGTLDIHKE
LEDLMAEFLNVEAVMSFGMGFATNAMNIPVFVGKGCLILSDEFNHTSVILGSRLSGAVIRPFKHNNAENL
EKLLREAIIRGQPGTGRAWKKILIVVEGVYSMEGSIVNLAQIVALKKKYKAYLYIDEAHSIGCTGPTGRG
VRELFGLDPEDIDVYMGTFTKSFSGSGGYIGGKKEIVDYLRMQSHSTTYATSMSPVVAAQLIRSLKITMD
MRGTLEVWREYSNLRKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175467
ORF Size 1311 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 2759 bp
RefSeq ORF 1692 bp
Locus ID 228677
UniProt ID Q8BG54
Cytogenetics 2 F3
MW 49.4 kDa
Summary Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. SPT complexes containing SPTLC3 generate shorter chain sphingoid bases compared to complexes containing SPTLC2. The SPTLC1-SPTLC3-SPTSSA isozyme uses C12-CoA, C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. On the other hand, the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.UniProtKB/Swiss-Prot Function
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"NM_175467" in other vectors (5)
SKU Description Size Price
MC219046 Sptlc3 (untagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3), (10ug) 10 ug
$577.00
MR206971L1 Lenti ORF clone of Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L2 Lenti ORF clone of Sptlc3 (mGFP-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L3 Lenti ORF clone of Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L4 Lenti ORF clone of Sptlc3 (mGFP-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00

Plasmid Map for MR206971

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206971
Circular map for MR206971

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