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Sptlc3 (NM_175467) Mouse Untagged Clone

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SKU
MC219046
Sptlc3 (untagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $577.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sptlc3
Synonyms C130053K05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219046 representing NM_175467
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAATCTGAACGACAGTGCTGTTACAAATGGGACTCTACACAATCCTAAGACCCAGCAAGGAAAAA
GGCAAAGCACTGGTTGTGTGAAAAATGGAATCTCAAAGGAAGCCCAGCAAAATAGGAAGGCATATGCTGA
GGATAAGCCAGTCTTTGAGCCCTACCAGGAAGCACCACTTTATGTCTACGTTTTAACTTACATGGGATAT
GGAATTGGAATCCTATTCGGCTATCTCAGAGACTTTATGAGAAACTGGGGAATTGAAAAATGCAATGCAG
CTGTAGAGCGAGAAGAACAAAAAGACTTTGTGCCGCTGTATCAGGACTTTGAGAACTTTTATAAGAGGAA
CCTTTACATGAGAATCAGAGACAGCTGGAGTCACACGGTCTGCAGTGCCCCAGAGCCTTACATGAATGTC
ATGGAGAAAGTGACTGATGACTATAACTGGACATTCAGGCATACTGGAAAAGTTATAGAAAATATCATCA
ACATGGCCTCCTACAACTACCTTGGGCTTGCAGGGAAATATGATGATTCGATGGTGAGAGTTAAGGATAC
TTTAGAAAAGTATGGTGTAGGTGTGGCCAGCACCAGAAATGAAATGGGTACCTTGGATATACACAAGGAG
TTGGAGGATCTCATGGCTGAGTTCTTGAATGTAGAAGCAGTGATGTCTTTTGGAATGGGATTCGCAACCA
ACGCAATGAATATCCCTGTATTTGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTTAACCACACTTC
AGTCATCCTAGGATCTCGACTCTCAGGTGCAGTCATAAGACCCTTCAAACACAATAATGCAGAAAACCTA
GAGAAGCTCCTGAGAGAAGCTATCATCCGTGGCCAGCCTGGCACAGGGAGAGCTTGGAAAAAGATTCTCA
TCGTGGTAGAGGGCGTCTACAGCATGGAAGGTTCCATTGTGAACCTTGCCCAGATAGTGGCTCTAAAGAA
GAAATACAAGGCTTACCTGTACATTGATGAAGCTCACAGTATTGGCTGTACAGGCCCCACGGGACGAGGC
GTCAGAGAGTTATTTGGACTGGACCCTGAAGATATTGACGTATACATGGGTACATTCACCAAGAGCTTCT
CGGGCTCAGGAGGATATATAGGCGGGAAAAAGGAAATTGTGGATTATTTACGGATGCAGTCACATAGCAC
TACTTATGCTACATCCATGAGTCCCGTAGTAGCAGCACAACTCATCAGATCATTGAAGATAACTATGGGA
TATGAGGGGAACATTGGAGGTATGGAGAGAATACAGCAACTTAAGGAAAACATAAAATACTTCCGACGGC
GACTGAAAGAGATGGGATTCATCATCTATGGCAATGATTTCTCTCCGGTGATCCCTGTGCTCCTCTATAT
GCCCGCTAAAGTGTCTGCTTTCAGCAGATTTTTGCTTAAGAAAAAGATCTCAGTGGTGGTTGTTGGATTT
CCAGCTACTTCCCTGCCAGAAGGTCGGGCCAGGTTCAGCATGTCCTCTGCACATACTCGGGAGATGTTAG
ACACAGTTTTGGAAGTCGTTGATGAGCTCGGTGATCTCCTGAACGTGAAATATTTCCCACTCAAGAAATC
AGGGCGTGCTATACTCTACAATAAGGAGGGTTTTGACAATGAAGCAAGCTTTGAAGAGATGCACTCTGAA
CCGGAAGCCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_175467
Insert Size 1692 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175467.3, NP_780676.1
RefSeq Size 2759 bp
RefSeq ORF 1692 bp
Locus ID 228677
UniProt ID Q8BG54
Cytogenetics 2 F3
Summary Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. SPT complexes containing SPTLC3 generate shorter chain sphingoid bases compared to complexes containing SPTLC2. The SPTLC1-SPTLC3-SPTSSA isozyme uses C12-CoA, C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. On the other hand, the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.
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Documents
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"NM_175467" in other vectors (5)
SKU Description Size Price
MR206971 Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$289.00 $457.00
MR206971L1 Lenti ORF clone of Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L2 Lenti ORF clone of Sptlc3 (mGFP-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L3 Lenti ORF clone of Sptlc3 (Myc-DDK-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00
MR206971L4 Lenti ORF clone of Sptlc3 (mGFP-tagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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