Sgk3 (BC018363) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206839
Sgk3 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sgk3
Synonyms 2510015P22Rik; A330005P07Rik; Cisk; fy; fz
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGAAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGAA
GAGCAGGATTGAATGAGTTCATTCAGAACTTGGTCAGATATCCAGAGCTTTACAACCATCCAGATGTCCG
AGCATTCCTTCAAATGGACAGCCCAAGACATCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTACT
TCGAAGCCACATTCTACCTCACGGAACATCAACCTGGGACCAACTGGAAATCCTCATGCTAAACCAACTG
ACTTCGATTTTTTAAAAGTTATTGGAAAGGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGA
TGGAAAATTTTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATT
ATGGCTGAACGCAATGTGCTCTTGAAAAATGTGAAGCACCCATTTTTGGTTGGATTGCACTATTCTTTCC
AAACAACTGAAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTCTTCTTTCACCTCCAAAG
GGAAAGGTCTTTTCCTGAACCCAGAGCGAGGTTTTATGCCGCGGAGATCGCCAGTGCCTTGGGCTACCTG
CACTCCATCAAAATAGTGTACAGAGACTTGAAGCCAGAAAATATTCTTTTGGATTCAATGGGACATGTTG
TCTTAACGGATTTTGGACTTTGCAAAGAAGGAATCGCTATTTCTGATACCACCACAACTTTTTGTGGTAC
ACCAGAGTACCTTGCACCTGAAGTAATCAGAAAACAGCCCTATGACAACACTGTGGACTGGTGGTGCCTG
GGCGCTGTTCTGTATGAGATGCTGTACGGGCTGCCTCCTTTTTACTGCCGAGATGTTGCTGAAATGTATG
ACAATATTCTTCACAAGCCCTTAAACTTGAGACCAGGAGTGAGTCTCACCGCCTGGTCCATTCTGGAAGA
ACTTCTAGAAAAAAACAGACAAAATCGACTTGGTGCCAAAGAAGACTTTCTTGAAATCCAGAATCATCCT
TTTTTTGAGTCACTCAGCTGGACTGACCTCGTACAAAAAAAGATTCCACCTCCATTTAACCCTAATGTGG
CTGGACCAGATGATATCAGAAACTTTGATGCCGTCTTCACTGAAGAAACGGTTCCCTATTCAGTGTGTGT
GTCTTCTGACTATTCCATCGTGAATGCCAGTGTTCTGGAGGCAGATGATGCATTTGTTGGTTTTTCTTAC
GCCCCTCCTTCGGAAGACTTATTTTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206839 protein sequence
Red=Cloning site Green=Tags(s)

MALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPRHQSDPSEDEDERST
SKPHSTSRNINLGPTGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHI
MAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEPRARFYAAEIASALGYL
HSIKIVYRDLKPENILLDSMGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCL
GAVLYEMLYGLPPFYCRDVAEMYDNILHKPLNLRPGVSLTAWSILEELLEKNRQNRLGAKEDFLEIQNHP
FFESLSWTDLVQKKIPPPFNPNVAGPDDIRNFDAVFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSY
APPSEDLFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC018363
ORF Size 1287 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018363, AAH18363
RefSeq Size 2494 bp
RefSeq ORF 1289 bp
Locus ID 170755
Cytogenetics 1 2.08 cM
MW 91.4 kDa
Summary Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC018363" in other vectors (4)
SKU Description Size Price
MC218544 Sgk3 (untagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682), (10ug) 10 ug
$503.00
MG206839 Sgk3 (tGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) 10 ug
$657.00
MR206839L3 Lenti ORF clone of Sgk3 (Myc-DDK-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) 10 ug
$757.00
MR206839L4 Lenti ORF clone of Sgk3 (mGFP-tagged) - Mouse serum/glucocorticoid regulated kinase 3 (cDNA clone MGC:19373 IMAGE:2631682) 10 ug
$757.00

Plasmid Map for MR206839

Download sequence for

Cloning scheme for MR206839

Circular map for MR206839

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products