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Aup1 (BC016485) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206456
Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Aup1 or BC016485"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aup1
Synonyms AA589454
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206456 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCTCCCGAGTGACGGCTTCC
TGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTGCGACTCTTCCTCGGGCT
CCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCGTGGTACGAACCATGTGT
GCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCGCGTCAGGGTCCTTATTT
CCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGTAGCACCCCTCTACTCAA
TAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGCGGGTGGAGTTGGTGGAG
TCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCTCTTCCCCGAGGAAGAGG
CCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATTCAGGACGTGGTACAACC
TCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCTCCTGGGTCTCAGAACTG
CTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCCCATTCGTCGACAGCTAG
GGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAATTGGGCCAGATAGGGAC
ACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCAGATTACGCCCCCAGTCA
GTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGACCACTCTCGCTCACAGAG
TCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCCAGGACTGGGTGTGTAGA
CTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATGTCACTGAGGGATCTCAG
TCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCCTCAGCCCACAGCCCTAA
CGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAGGCACTGTATGAATATGC
AAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206456 protein sequence
Red=Cloning site Green=Tags(s)

MEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVLRLFLGLHVFLVSCALPDSVLRRFVVRTMC
AVLGLVARQEDSGLRDHRVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMDRRVELVE
SLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSEL
LWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKELGQIGTRLTPADKAEHMKRQRHPRLRPQS
VQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLARTGCVDLTITNLLEGAVAFMPEDVTEGSQ
SPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQALYEYARRRFRERQAQEAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC016485
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016485, AAH16485
RefSeq Size 1454 bp
RefSeq ORF 1232 bp
Locus ID 11993
Cytogenetics 6 35.94 cM
MW 46.1 kDa
Summary The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2014
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"BC016485" in other vectors (4)
SKU Description Size Price
MC218140 Aup1 (untagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982), (10ug) 10 ug
$503.00
MG206456 Aup1 (tGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$657.00
MR206456L3 Lenti ORF clone of Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$757.00
MR206456L4 Lenti ORF clone of Aup1 (mGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$757.00

Plasmid Map for MR206456

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206456
Circular map for MR206456

file-alt Citations

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