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Aup1 (BC016485) Mouse Untagged Clone

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SKU
MC218140
Aup1 (untagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Aup1
Synonyms AA589454
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC016485
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCTCCCGAGTGACGGCTTCC
TGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTGCGACTCTTCCTCGGGCT
CCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCGTGGTACGAACCATGTGT
GCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCGCGTCAGGGTCCTTATTT
CCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGTAGCACCCCTCTACTCAA
TAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGCGGGTGGAGTTGGTGGAG
TCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCTCTTCCCCGAGGAAGAGG
CCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATTCAGGACGTGGTACAACC
TCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCTCCTGGGTCTCAGAACTG
CTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCCCATTCGTCGACAGCTAG
GGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAATTGGGCCAGATAGGGAC
ACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCAGATTACGCCCCCAGTCA
GTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGACCACTCTCGCTCACAGAG
TCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCCAGGACTGGGTGTGTAGA
CTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATGTCACTGAGGGATCTCAG
TCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCCTCAGCCCACAGCCCTAA
CGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAGGCACTGTATGAATATGC
AAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC016485
Insert Size 1233 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016485, AAH16485
RefSeq Size 1454 bp
RefSeq ORF 1232 bp
Locus ID 11993
Cytogenetics 6 35.94 cM
Summary The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2014
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"BC016485" in other vectors (4)
SKU Description Size Price
MG206456 Aup1 (tGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$657.00
MR206456 Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$289.00 $457.00
MR206456L3 Lenti ORF clone of Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$757.00
MR206456L4 Lenti ORF clone of Aup1 (mGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) 10 ug
$757.00

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