Nfe2 (NM_008685) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nfe2 |
| Synonyms | NF-E2; NF-E2/P45; p45; p45nf-e2; p45NFE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008685 |
| ORF Size | 1119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008685.3, NP_032711.2 |
| RefSeq Size | 1793 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 18022 |
| UniProt ID | Q07279 |
| Cytogenetics | 15 58.62 cM |
| MW | 42 kDa |
| Summary | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008685" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209025 | Nfe2 (untagged) - Mouse nuclear factor, erythroid derived 2 (Nfe2), (10ug) | 10 ug |
$503.00
|
|
| MG205777 | Nfe2 (tGFP-tagged) - Mouse nuclear factor, erythroid derived 2 (Nfe2) | 10 ug |
$657.00
|
|
| MR205777L3 | Lenti ORF clone of Nfe2 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2 (Nfe2) | 10 ug |
$757.00
|
|
| MR205777L4 | Lenti ORF clone of Nfe2 (mGFP-tagged) - Mouse nuclear factor, erythroid derived 2 (Nfe2) | 10 ug |
$757.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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