Vps72 (NM_009336) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205675
Vps72 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vps72
Synonyms Tcfl1; YL-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205675 representing NM_009336
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTTGGCTGGGGGCCGGGCCCCACGGAAAACCGCTGGGAACCGGCTTTCTGGGCTCCTGGAAGCAG
AGGAGGAAGATGAGTTCTACCAGACAACTTACGGGGGTTTCACCGAGGAATCTGGAGATGATGAGTATCA
AGGAGACCAGTCTGATACGGAGGATGAGGTAGACTCTGACTTTGACATTGATGAAGGGGATGAACCATCC
AGTGATGGAGAAGCGGAAGAGCCAAGGAGGAAGCGCAGAGTCGTCACCAAAGCCTATAAGGAGCCTCTAA
AGAGCTTGAGGCCTCGCAAGGTCAGCACCCCAGCAAGCAGCTCCCAGAAGGCCAGAGAGGAGAAGACGCT
GCTGCCACTGGAGCTCCAGGACGATGGCTCTGACAGCCGCAAGTCTATGCGTCAGTCTACAGCTGAGCAC
ACACGACAAACGTTCCTTCGGGTTCAAGAGAGGCAAGGACAGTCCCGGAGGCGGAAGGGTCCCCACTGTG
AGCGACCACTAACCCAGGAAGAGCTGCTCAGGGAGGCCAAGATCACTGAAGAGCTCAACTTACGTTCACT
CGAGACCTATGAAAGGCTGGAGGCTGACAAAAAGAAGCAGGTTCACAAGAAGCGGAAGTGCCCTGGGCCA
ATAATCACCTACCACTCAGTGACGGTGCCGCTTGTTGGGGAGCCCGGCCCCAAGGAGGAAAATGTGGACG
TGGAAGGCCTTGATCCTGCTCCTACAGCCTCTGCACTGGCTCCACATGCTGGGACCGGGACTGGAGCTGC
CGCTGCCACCCCTCCTGCTCACTGCTCTCGCACCTTCATCACGTTCAGTGATGATGCAACGTTTGAAGAG
TGGTTCCCCCAGGGGCGACCCCCGAAGGTCCCGGTGCGGGAGGTCTGCCCAGTGACCCACCGTCCAGCTT
TGTACCGGGACCCTGTTACAGACATACCCTATGCCACTGCTAGAGCCTTCAAGATCATCCGTGAGGCGTA
CAAGAAGTACATCACTGCCCACGGACTTCCACCCACCGCCTCGGCGCTGGGCCCCGGGCCCCCACCCCCC
GAGCCACTACCTGGCTCTGGGCCTCGAGCCTTGCGTCAGAAGATTGTCATCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205675 representing NM_009336
Red=Cloning site Green=Tags(s)

MSLAGGRAPRKTAGNRLSGLLEAEEEDEFYQTTYGGFTEESGDDEYQGDQSDTEDEVDSDFDIDEGDEPS
SDGEAEEPRRKRRVVTKAYKEPLKSLRPRKVSTPASSSQKAREEKTLLPLELQDDGSDSRKSMRQSTAEH
TRQTFLRVQERQGQSRRRKGPHCERPLTQEELLREAKITEELNLRSLETYERLEADKKKQVHKKRKCPGP
IITYHSVTVPLVGEPGPKEENVDVEGLDPAPTASALAPHAGTGTGAAAATPPAHCSRTFITFSDDATFEE
WFPQGRPPKVPVREVCPVTHRPALYRDPVTDIPYATARAFKIIREAYKKYITAHGLPPTASALGPGPPPP
EPLPGSGPRALRQKIVIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009336
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009336.2, NP_033362.2
RefSeq Size 1436 bp
RefSeq ORF 1107 bp
Locus ID 21427
UniProt ID Q62481
Cytogenetics 3 F2.1
MW 41.2 kDa
Summary Deposition-and-exchange histone chaperone specific for H2AFZ, specifically chaperones H2AFZ and deposits it into nucleosomes. As component of the SRCAP complex, mediates the ATP-dependent exchange of histone H2AFZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009336" in other vectors (4)
SKU Description Size Price
MC200434 Vps72 (untagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72), (10ug) 10 ug
$457.00
MG205675 Vps72 (tGFP-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72) 10 ug
$657.00
MR205675L3 Lenti ORF clone of Vps72 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72) 10 ug
$757.00
MR205675L4 Lenti ORF clone of Vps72 (mGFP-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72) 10 ug
$757.00

Plasmid Map for MR205675

Download sequence for

Cloning scheme for MR205675

Circular map for MR205675

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