Scd2 (NM_009128) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205478
Scd2 (Myc-DDK-tagged) - Mouse stearoyl-Coenzyme A desaturase 2 (Scd2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scd2
Synonyms mir-5114; Mir5114; Scd-2; swty
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205478 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCCACATACTGCAAGAGATCTCTGGCGCTTACTCAGCCACCACCACAATCACAGCGCCACCTT
CTGGGGGACAGCAGAATGGAGGCGAGAAGTTTGAAAAGAGTTCTCACCACTGGGGAGCAGATGTTCGCCC
TGAACTAAAAGATGATCTATATGACCCCACCTATCAGGATGATGAGGGGCCCCCGCCCAAGCTGGAGTAC
GTCTGGAGGAACATCATTCTCATGGCCCTGCTGCATTTGGGAGCCTTGTACGGGATCACACTGGTTCCCT
CCTGCAAGCTCTACACCTGTCTCTTCGCGTATTTGTACTATGTAATCAGCGCCCTGGGCATCACAGCCGG
GGCTCATCGCCTGTGGAGCCACAGAACATACAAGGCACGGCTGCCCCTGAGGCTCTTCCTCATCATTGCC
AACACCATGGCGTTCCAGAATGACGTGTATGAATGGGCCCGAGATCACCGCGCCCACCACAAGTTCTCAG
AAACACACGCCGACCCTCACAATTCCCGCCGTGGCTTCTTTTTCTCTCACGTGGGTTGGCTGCTTGTGCG
CAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGTCTGACCTGAAAGCCGAGAAGCTGGTG
ATGTTCCAGAGGAGGTACTACAAGCCCGGCCTCCTGCTGATGTGCTTCGTCCTGCCCACGCTGGTGCCCT
GGTACTGCTGGGGCGAGACTTTTGTAAACAGCCTGTGCGTTAGCACCTTCTTGCGATACGCCGTGGTGCT
CAACGCCACCTGGCTGGTGAACAGTGCCGCCCACCTCTACGGATATCGCCCCTACGACAAGAACATTAGC
TCTCGGGAGAACATCTTGGTTTCCATGGGAGCTGTGGGCGAGGGCTTCCACAACTACCACCACGCCTTCC
CCTACGACTACTCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGATTGCATGGC
TCTCCTGGGCCTGGCTTACGACCGGAAGAGAGTGTCCAGGGCTGCTGTCTTGGCCAGGATTAAGAGAACT
GGAGACGGAAGCTGCAAGAGCGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205478 protein sequence
Red=Cloning site Green=Tags(s)

MPAHILQEISGAYSATTTITAPPSGGQQNGGEKFEKSSHHWGADVRPELKDDLYDPTYQDDEGPPPKLEY
VWRNIILMALLHLGALYGITLVPSCKLYTCLFAYLYYVISALGITAGAHRLWSHRTYKARLPLRLFLIIA
NTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLV
MFQRRYYKPGLLLMCFVLPTLVPWYCWGETFVNSLCVSTFLRYAVVLNATWLVNSAAHLYGYRPYDKNIS
SRENILVSMGAVGEGFHNYHHAFPYDYSASEYRWHINFTTFFIDCMALLGLAYDRKRVSRAAVLARIKRT
GDGSCKSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009128
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009128.2, NP_033154.2
RefSeq Size 5453 bp
RefSeq ORF 1077 bp
Locus ID 20250
UniProt ID P13011
Cytogenetics 19 37.98 cM
MW 40.9 kDa
Summary Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates (PubMed:16443825). Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA (PubMed:16443825). Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids (PubMed:16443825). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides, especially during embryonic development and in neonates (PubMed:16118274). Important for normal permeability barrier function of the skin in neonates (PubMed:16118274).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009128" in other vectors (4)
SKU Description Size Price
MC207388 Scd2 (untagged) - Mouse stearoyl-Coenzyme A desaturase 2 (Scd2), (10ug) 10 ug
$503.00
MG205478 Scd2 (tGFP-tagged) - Mouse stearoyl-Coenzyme A desaturase 2 (Scd2) 10 ug
$489.00 $657.00
MR205478L3 Lenti ORF clone of Scd2 (Myc-DDK-tagged) - Mouse stearoyl-Coenzyme A desaturase 2 (Scd2) 10 ug
$757.00
MR205478L4 Lenti ORF clone of Scd2 (mGFP-tagged) - Mouse stearoyl-Coenzyme A desaturase 2 (Scd2) 10 ug
$757.00

Plasmid Map for MR205478

Download sequence for

Cloning scheme for MR205478

Circular map for MR205478

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