Prkaca (NM_008854) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR205322
Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca)
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Prkaca |
| Synonyms | C; P; Pk; Pkaca; PKCD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008854 |
| ORF Size | 1053 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008854.5 |
| RefSeq Size | 2292 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 18747 |
| UniProt ID | P05132 |
| Cytogenetics | 8 C2 |
| MW | 41 kDa |
| Summary | This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. provided by RefSeq, Apr 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008854" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200197 | Prkaca (untagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca), (10ug) | 10 ug |
$457.00
|
|
| MG205322 | Prkaca (tGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) | 10 ug |
$886.00
|
|
| MR205322L3 | Lenti ORF clone of Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) | 10 ug |
$986.00
|
|
| MR205322L4 | Lenti ORF clone of Prkaca (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) | 10 ug |
$986.00
|
|
Citations
Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice
Yin, Zhang, Zhou et al
Proc Natl Acad Sci U S A (2025) 122 (4), e2418317122 DOI: 10.1073/pnas.2418317122
Yin, Zhang, Zhou et al
Proc Natl Acad Sci U S A (2025) 122 (4), e2418317122 DOI: 10.1073/pnas.2418317122
Cited by 14
AI Summary: OriGene products contributed to this study by providing mouse open reading frame (ORF) plasmids for CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), and PKAα (MR205322), which were used to investigate the roles of these proteins in regulating non-rapid eye movement sleep in mice.
Snippet: Cas9;Sik3Slp-flox/flox with CMV-Cre/+ mice (006054, JAX). Plasmids. Mouse open reading frames (ORFs) of CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), and PKAα (MR205322) were purchased from OriGene Technologies. pAAV-Tre-3HA-CnAα/β/γCA were constructed from pAAV-Tre-EGFP by replacing EGFP with ORF sequences of CnAαCA (AA 1-398), CnAβCA (AA 1-408), and CnAγCA (AA 1-391) mutants lack
Calcineurin is a central regulator of Process S (sleep homeostasis) in mice
Liu, Yin, Zhang et al
Research Square (2023)
Liu, Yin, Zhang et al
Research Square (2023)
Snippet: Mouse open reading frames (ORFs) of CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), PKAα (MR205322) were purchased from OriGene Technologies. pAAV-hSyn-3HACnAα/β/γ* or pAAV-Tre-3HA-CnAα/β/γ* mutants were constructed from pAAV-hSyn-EGFP (Addgene, 105539) or pAAV-Tre-EGFP by replacing GFP with DNA sequences of CnAα (AA 1
Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis
Luo, Zhu, Ren et al
Front Oncol (2022) 12, 1036543 DOI: 10.3389/fonc.2022.1036543
Luo, Zhu, Ren et al
Front Oncol (2022) 12, 1036543 DOI: 10.3389/fonc.2022.1036543
Cited by 32
AI Summary: OriGene products contributed to this study by providing the HIF-1α-Flag (MR210895) and PRKACA (MR205322) expression vectors, which were used to investigate the molecular mechanisms underlying lactate-induced tumor extracellular vesicle production.
Snippet: outh Junction, NJ, USA). Anti-CD9 antibody (MZ3) and CD63 antibody (NVG-2) were purchased from BioLegend (San Diego, CA, USA). HIF-1α-Flag (MR210895) and PRKACA (MR205322) vectors were obtained from Origene Technologies (Rockwell, Maryland, USA). Human samples Human lung tissue samples from lung cancer patients and Para-Ca were obtained from the Affiliated Hangzhou First People’s Hospital, Zhe
Ursodeoxycholic acid reduces antitumor immunosuppression by inducing CHIP-mediated TGF-β degradation
Shen, Lu, Song et al
Nat Commun (2022) 13 (1), 3419 DOI: 10.1038/s41467-022-31141-6
Shen, Lu, Song et al
Nat Commun (2022) 13 (1), 3419 DOI: 10.1038/s41467-022-31141-6
Cited by 112
AI Summary: OriGene products, specifically the plasmids PCMV6-TGF-β1-Myc-Flag (MR227339), PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322), and PCMV6-p62-Myc-Flag (MR226105), were used in this study to investigate the molecular mechanisms by which ursodeoxycholic acid promotes TGF-β degradation and enhances antitumor immunity.
Snippet: … The PCMV6-TGF-β1-Myc-Flag (MR227339), PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322) and PCMV6-p62-Myc-Flag (MR226105) plasmids were purchased from Origene Technologies (Rockwell, Maryland, USA). The PCMV6-TGF-β1-Flag, TGF-β1 mutant, PCMV6-CHIP-Flag, PCMV6-CHIP-His, PCMV6-PRKACA-Myc, pMX-PRKACA and PCMV6-p62_UBAΔ-Myc plasmids were constructed;, PCMV6-CHIP-Myc-Flag (MR204258), PCMV6-PRKACA-Myc-Flag (MR205322) and PCMV6-p62-Myc-Flag (MR226105) plasmids were purchased from Origene Technologies (Rockwell, Maryland, USA)
Showing 1-4 of 4 papers.
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