OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Prkaca (NM_008854) Mouse Untagged Clone

Be the first to review this product
SKU
MC200197
Prkaca (untagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Prkaca or NM_008854"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prkaca
Synonyms C; P; Pk; Pkaca; PKCD
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC003238 sequence for NM_008854
CTTGGGCTGAGGCTCCCCCGCGGGCGGGCGCAGAGAGACGCGGGAAGCAGGGGCTGGGCGGGGGTCGTGG CGCCGCAGCCAGCGCAGCCAGCCCCAGGGGCCGCCGCCTCCGCTGCCCAGCGCGCTCCGGGGCCGCCGGC CACCTTAGCACCCGCCGCGTCGCAGCTCCGGGACTGGCCCCGGCCGCGACGCCGCCGCGATGGGCAACGC CGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAGTTCCTAGCCAAAGCCAAGGAAGATTTC CTGAAAAAATGGGAGACCCCTTCTCAGAATACAGCCCAGTTGGATCAGTTTGATAGAATCAAGACCCTTG GCACCGGCTCCTTTGGGCGAGTGATGCTGGTGAAGCACAAGGAGAGTGGGAACCACTACGCCATGAAGAT CTTAGACAAGCAGAAGGTGGTGAAGCTAAAGCAGATCGAGCACACTCTGAATGAGAAGCGCATCCTGCAG GCCGTCAACTTCCCGTTCCTGGTCAAACTTGAATTCTCCTTCAAGGACAACTCAAACCTGTACATGGTCA TGGAGTATGTAGCTGGTGGCGAGATGTTCTCCCACCTACGGCGGATTGGAAGGTTCAGCGAGCCCCATGC CCGTTTCTACGCGGCGCAGATCGTCCTGACCTTTGAGTATCTGCACTCCCTGGACCTCATCTACCGGGAC CTGAAGCCCGAGAATCTTCTCATCGACCAGCAGGGCTATATTCAGGTGACAGACTTCGGTTTTGCCAAGC GTGTGAAAGGCCGTACTTGGACCTTGTGTGGGACCCCTGAGTACTTGGCCCCCGAGATTATCCTGAGCAA AGGCTACAACAAGGCTGTGGACTGGTGGGCTCTCGGAGTCCTCATCTACGAGATGGCTGCTGGTTACCCA CCCTTCTTCGCTGACCAGCCTATCCAGATCTATGAGAAAATCGTCTCTGGGAAGGTGCGGTTCCCATCCC ACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTTCTGCAGGTGGATCTCACCAAGCGCTTTGGGAA CCTCAAGAACGGGGTCAATGACATCAAGAACCACAAGTGGTTTGCCACGACTGACTGGATTGCCATCTAT CAGAGAAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGACACGAGTAACTTTGACGACT ATGAGGAGGAAGAGATCCGGGTCTCCATCAATGAGAAGTGTGGCAAGGAGTTTACTGAGTTTTAGGGGTG TGCTTGTGCCCCTTGGGTTCTCTTTCATTTTTTCTTTTTCTTTCTATTTTTTTTCCGGTTGGGGGTGGGA GGGTTGGATCGAACAGCCAGAGGGCCCTAGAGTTCCATGCATCTAATTTAACATCCACTCCACACCCCCA GGGTTAAGGAGAGCAGGAAAGCGCTCCAGATACTGGGGAAGGGGCAACATCAGCTGCTCCCCCTCTCCCT TCTTCTCCACCCTTCCCTGCCTGTTTTCAATGAATTTCTTAGCTCCAGCCATACCCAATCTTGCTGGTGT ATCCAGGGGCAGGGTACGGAAAGAGGGCCCCAAATTCAGCCTCCTTCCCGACCCTAGCACTGGATACTAA GGATGAACGAACAGTAACGCCAACCTTCCCTTCCATGCAGCCCTACCTGGAAAGGGAGATTTTATGACCT GTACAGAGGGCTGCTTGCCAGTGGGGTTTTTTTTTTTTTTTCATTTAAATTAAGTTCCACCAGTGCCTCC CACCCTCCAAACTGTCCCACCCTCCCCAAACACCCTCCTCACTCCCTAAATCCATTCTGATGAGACCCGG GTAGCCAACTGACCCTGTCAAGGAAGGAACTGGGCTTGGAATCTCGCCCTGAGCTGCTAGCCTCCCGGCC CCCCTTTCCAGTGGTCTCATGCCAATTGTCCTGTGCATCAGCCCCCTTAAGAAGCCTCCCCCATCCTGGG CGCCTCGCTTCTAGCTTAGCTGTCAGCTGTCCATCACCTCTTGCCGTGCGTCCCCACTCACTGCAACCCC AAGTCTGATTGTGCTTTTTCTCTCAATAGAAAGGTGGGGAGCTGCTGGGGAAATTACCCCATTTATCCCT GTGTTTATCCCTCTCGTAACTTCTCCCAAAAAGGAGGAGCTCTCAGGCCTGGGTGGGGGCCCCGGGTGGA CGAGGGGGTCGTTCAACCTGTGTGCTTCGAAGGATGAGACTTCCTCTTGAACAGTGTGCTGTTGTAAACA TATTTGAAAACTATTACCAATAAAGTTTTGTTTTAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008854
Insert Size 1056 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003238, AAH03238
RefSeq Size 2292 bp
RefSeq ORF 1056 bp
Locus ID 18747
UniProt ID P05132
Cytogenetics 8 C2
Summary This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. provided by RefSeq, Apr 2013
Transcript Variant: This variant (1, also known as Calpha1) represents the longer transcript and encodes the longer isoform (1).
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_008854" in other vectors (4)
SKU Description Size Price
MG205322 Prkaca (tGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) 10 ug
$886.00
MR205322 Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) 10 ug
$686.00
MR205322L3 Lenti ORF clone of Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) 10 ug
$986.00
MR205322L4 Lenti ORF clone of Prkaca (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) 10 ug
$986.00

file-alt Citations

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
Ding, Li, Enterina et al
Nat. Methods (2015)
Cited by 162
AI Summary: OriGene contributed to this study by providing the plasmid encoding the catalytic subunit of PKA (MC200197), which was used to construct the cAMP-dependent PKA biosensor.
Snippet: …To construct the cAMP-dependent PKA biosensor, we fused the gene encoding RA to the 5′ end of the catalytic subunit of PKA, and the gene encoding B was fused to the 3′ end of the regulatory subunit. Plasmids encoding the catalytic and regulatory subunits of PKA were obtained from OriGene (MC200197) and Source Bioscience (IRATp970F0898D), respectively. …
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products