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Aifm2 (NM_153779) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205050
Aifm2 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aifm2
Synonyms 5430437E11Rik; Amid; D730001I10Rik; PRG3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205050 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCCCAGGTCTCGGTGGATACAGGAGCCGTGCACGTGGTGATCGTGGGCGGGGGCTTCGGAGGGA
TAGCGGCTGCCAGCCAGCTGCAGGCGCTGAATGTCCCCTTCATGCTGGTGGATATGAAGGACTCCTTCCA
CCACAATGTGGCAGCCCTCCGGGCCTCCGTGGAGAGCGGGTTCGCCAAAAAGACATTCATTTCGTACTCT
GCGACCTTCAAGGACAACTTCCGCCAGGGCAAAGTGATTGGCATAGACTTGAAGAACCGGATGGTGTTGC
TACAGGGTGGCGAGGCCTTGCCCTTCTCACATCTTATCCTGGCCACAGGCAGCACCGGACCCTTCCCTGG
CAAGTTTAACGAGGTGTCCTGCCAGCAGGCAGCCATCCAGGCCTATGAGGACATGGTGAAGCAGATCCAG
CGCTCACAATTCATCGTGGTGGTGGGAGGCGGCTCTGCAGGAGTAGAGATGGCAGCAGAGATTAAAACCG
AGTACCCTGAGAAGGAGGTCACTCTTATCCACTCCAGAGTACCCCTGGCCGACAAGGAACTCCTGCCCTG
TGTGCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGTGTGCAGCTGCTGCTGAGTGAGCGGGTGAGC
AACCTGGAGGAACTGCCTCGCAATGAGTATCGGGAGTACATCAAGGTGGAGACAGACAAGGGCACGGAGG
TGGCCACCAACATGGTGATTGTGTGCAATGGGATCAAGATCAACAGCTCTGCCTACCGCAGTGCATTTGA
GAGTAGGCTGGCTAGCAATGGTGCTCTGAAAGTGAACGAGTTCCTCCAGGTGGAAGGTTACAGCAATATT
TATGCCATTGGTGACTGTGCCGATACCAAGGAGCCCAAGATGGCCTACCACGCTGGCCTGCATGCCAATG
TTGCCGTGGCCAACATCGTCAACTCCATGAAGCAGAGGCCACTCAAAGCTTACAAGCCAGAGACTGACCA
ACCACCGGCAGCTTTGTCCCCGGCTCTGCTGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205050 protein sequence
Red=Cloning site Green=Tags(s)

MGSQVSVDTGAVHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVESGFAKKTFISYS
ATFKDNFRQGKVIGIDLKNRMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSCQQAAIQAYEDMVKQIQ
RSQFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSRVPLADKELLPCVRQEVKEILLRKGVQLLLSERVS
NLEELPRNEYREYIKVETDKGTEVATNMVIVCNGIKINSSAYRSAFESRLASNGALKVNEFLQVEGYSNI
YAIGDCADTKEPKMAYHAGLHANVAVANIVNSMKQRPLKAYKPETDQPPAALSPALLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153779
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153779.1
RefSeq Size 1351 bp
RefSeq ORF 1143 bp
Locus ID 71361
UniProt ID Q8BUE4
Cytogenetics 10 B4
MW 36.7 kDa
Summary Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death (By similarity). May contribute to genotoxin-induced growth arrest.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153779" in other vectors (3)
SKU Description Size Price
MC207705 Aifm2 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) 10 ug
$503.00
MR205050L3 Lenti ORF clone of Aifm2 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$757.00
MR205050L4 Lenti ORF clone of Aifm2 (mGFP-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$757.00

Plasmid Map for MR205050

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205050
Circular map for MR205050

file-alt Citations

The origin of hepatocellular carcinoma depends on metabolic zonation
Guo, Liang, Chung et al
Science (2025)
Cited by 36
AI Summary: In this study, the authors used Fsp1 cDNA from OriGene (#MR205050) to investigate the metabolic determinants of hepatocellular carcinoma initiation in different liver zones.
Snippet: ctor px333 (Addgene #64073). cDNA for GFP and Luciferase were synthesized by GENEWIZ, and cDNA for Gstm3 were synthesized from Genscript. Fsp1 cDNA was purchased from Origene (#MR205050). Gateway sites were added to the cDNA through PCR, and genes of interest wer
Showing 1-1 of 1 papers.
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