Aifm2 (NM_153779) Mouse Untagged Clone

SKU
MC207705
Aifm2 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)
  $503.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Aifm2
Synonyms 5430437E11Rik; Amid; D730001I10Rik; PRG3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207705 representing NM_153779
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCCCAGGTCTCGGTGGATACAGGAGCCGTGCACGTGGTGATCGTGGGCGGGGGCTTCGGAGGGA
TAGCGGCTGCCAGCCAGCTGCAGGCGCTGAATGTCCCCTTCATGCTGGTGGATATGAAGGACTCCTTCCA
CCACAATGTGGCAGCCCTCCGGGCCTCCGTGGAGAGCGGGTTCGCCAAAAAGACATTCATTTCGTACTCT
GCGACCTTCAAGGACAACTTCCGCCAGGGCAAAGTGATTGGCATAGACTTGAAGAACCGGATGGTGTTGC
TACAGGGTGGCGAGGCCTTGCCCTTCTCACATCTTATCCTGGCCACAGGCAGCACCGGACCCTTCCCTGG
CAAGTTTAACGAGGTGTCCTGCCAGCAGGCAGCCATCCAGGCCTATGAGGACATGGTGAAGCAGATCCAG
CGCTCACAATTCATCGTGGTGGTGGGAGGCGGCTCTGCAGGAGTAGAGATGGCAGCAGAGATTAAAACCG
AGTACCCTGAGAAGGAGGTCACTCTTATCCACTCCAGAGTACCCCTGGCCGACAAGGAACTCCTGCCCTG
TGTGCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGTGTGCAGCTGCTGCTGAGTGAGCGGGTGAGC
AACCTGGAGGAACTGCCTCGCAATGAGTATCGGGAGTACATCAAGGTGGAGACAGACAAGGGCACGGAGG
TGGCCACCAACATGGTGATTGTGTGCAATGGGATCAAGATCAACAGCTCTGCCTACCGCAGTGCATTTGA
GAGTAGGCTGGCTAGCAATGGTGCTCTGAAAGTGAACGAGTTCCTCCAGGTGGAAGGTTACAGCAATATT
TATGCCATTGGTGACTGTGCCGATACCAAGGAGCCCAAGATGGCCTACCACGCTGGCCTGCATGCCAATG
TTGCCGTGGCCAACATCGTCAACTCCATGAAGCAGAGGCCACTCAAAGCTTACAAGCCAGAGACTGACCA
ACCACCGGCAGCTTTGTCCCCGGCTCTGCTGCTGTGGACACCAGCCAGGAAGCTCACCCTCTCTGAGGGA
AGGATCAACTACCTGCAGGAAGCCGGCCATGTGAGAACTCTACAGAGCATGGCGGGACTCTTCAGTGACA
GGCCTTGTGTTATTTCTTTATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_153779
Insert Size 1143 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153779.2, NP_722474.2
RefSeq Size 1351 bp
RefSeq ORF 1143 bp
Locus ID 71361
UniProt ID Q8BUE4
Cytogenetics 10 B4
Summary Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death (By similarity). May contribute to genotoxin-induced growth arrest.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) has an alternate 5' UTR exon and lacks the penultimate exon, resulting in a downstream stop codon, compared to variant 4. The resulting isoform (1) is longer and has a distinct C-terminus, compared to isoform 2.
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Documents
Resources
"NM_153779" in other vectors (3)
SKU Description Size Price
MR205050 Aifm2 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$289.00 $457.00
MR205050L3 Lenti ORF clone of Aifm2 (Myc-DDK-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$757.00
MR205050L4 Lenti ORF clone of Aifm2 (mGFP-tagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$757.00

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