Ubiad1 (NM_027873) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204986
Ubiad1 (Myc-DDK-tagged) - Mouse UbiA prenyltransferase domain containing 1 (Ubiad1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ubiad1
Synonyms 1200002M06Rik; AI426463; AW320947; Tere1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204986 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGTACAGGCCCCGGGGGAGAAGATTAATATCCTGGCAGGAGAGACAGCCAAGGTCGGGGACC
CGCAGAAGAACGAATGGCCCGAGCAGGACAGGTTTCCTGAACGATCCTGGAGGCACAAGTGCGCCTCCTA
CGTGTTGGCCCTGAGGCCCTGGAGCTTCAGTGCCTCACTCACCCCTGTGGCCCTGGGCAGTGCCTTGGCC
TACAGGTCTCAGGGTGTCCTGGATCCCAGGCTGTTGTTGGGTTGTGCAGTGGCTGTCCTGGCTGTGCACG
GGGCCGGCAATTTGGTCAACACATACTATGACTTTTCCAAGGGCATTGACCACAAAAAGAGTGATGACAG
AACTTTGGTGGACAGAATTCTGGAGCCCCAGGATGTTGTTCGATTTGGAGTCTTCCTCTACACCTTGGGC
TGCGTCTGTGCTGCTTGCCTCTACTACTTGTCCGCTCTGAAATTGGAACACTTGGCTCTCATCTACTTCG
GAGGCCTGTCTGGCTCCTTTCTCTACACAGGAGGAATTGGATTCAAGTATGTGGCCCTGGGAGACCTCGT
CATCCTCATCACTTTCGGCCCGCTGGCTGTGATGTTTGCCTACGCTGTCCAGGTGGGATCCCTGGCCATC
TTCCCTCTAATCTACGCCATCCCTCTGGCCCTCAGCACGGAGGCCATTCTCCATTCCAACAACACCAGGG
ACATGGAATCTGACCGAGAGGCTGGCATCGTCACGCTGGCCATCCTCATTGGGCCCACCTTCTCCTATGT
CCTCTATAACACACTGCTCTTTGTGCCCTACCTAATCTTTACCATCCTGGCCACGCACTGCAGCATCAGC
CTGGCACTGCCCCTGCTCACCATCCCCATGGCCTTCTCCCTTGAGAGGCAGTTCCGCAGCCAGGCCTTCA
ACAAGCTGCCCCAGAGGACAGCCAAGCTCAACCTCCTGCTGGGGCTTTTCTATGTCTTTGGCATCATCCT
GGCACCAGCAGGCAGCCTGCCCAGACTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR204986 protein sequence
Red=Cloning site Green=Tags(s)

MAAVQAPGEKINILAGETAKVGDPQKNEWPEQDRFPERSWRHKCASYVLALRPWSFSASLTPVALGSALA
YRSQGVLDPRLLLGCAVAVLAVHGAGNLVNTYYDFSKGIDHKKSDDRTLVDRILEPQDVVRFGVFLYTLG
CVCAACLYYLSALKLEHLALIYFGGLSGSFLYTGGIGFKYVALGDLVILITFGPLAVMFAYAVQVGSLAI
FPLIYAIPLALSTEAILHSNNTRDMESDREAGIVTLAILIGPTFSYVLYNTLLFVPYLIFTILATHCSIS
LALPLLTIPMAFSLERQFRSQAFNKLPQRTAKLNLLLGLFYVFGIILAPAGSLPRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027873
ORF Size 1008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027873.1, NM_027873.2, NP_082149.1
RefSeq Size 2970 bp
RefSeq ORF 1011 bp
Locus ID 71707
UniProt ID Q9DC60
Cytogenetics 4 E2
MW 36.7 kDa
Summary Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays an important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027873" in other vectors (3)
SKU Description Size Price
MC204868 Ubiad1 (untagged) - Mouse UbiA prenyltransferase domain containing 1 (Ubiad1), (10ug) 10 ug
$457.00
MR204986L3 Lenti ORF clone of Ubiad1 (Myc-DDK-tagged) - Mouse UbiA prenyltransferase domain containing 1 (Ubiad1) 10 ug
$757.00
MR204986L4 Lenti ORF clone of Ubiad1 (mGFP-tagged) - Mouse UbiA prenyltransferase domain containing 1 (Ubiad1) 10 ug
$757.00

Plasmid Map for MR204986

Download sequence for

Cloning scheme for MR204986

Circular map for MR204986

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