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Etfa (NM_145615) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204913
Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Etfa
Synonyms 2010200I21Rik; D9Ertd394e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204913 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGAGCAGCGGCGCCTGGGCAGCTCCGGCGGGCGGCCTCATTGCTCCGTTTTCAGAGTACCTTGG
TAATAGCTGAGCATGCAAATGATTCCCTAGCACCCATTACTCTAAATACTATCACTGCAGCTGGACGTCT
TGGAGGTGAAGTGTCCTGCTTAGTAGCTGGAACCAAGTGTGACAAGGTGGTACAGGATCTATGTAAAGTA
GCTGGCGTAGCAAAGGTTCTGGTGGCTCAGCATGATGCATACAAAGGCCTCCTTCCAGAGGAACTCACAC
CGTTGATTTTGGAAACTCAGAAGCAGTTCAGTTACACACACATCTGTGCTGGAGCGTCTGCTTTTGGAAA
GAACCTTCTGCCCAGAGTAGCTGCCAAACTTAATGTTGCTCCAGTTTCTGACATCATTGAGATCAAGTCA
CCTGACACATTTGTGAGAACTATCTATGCAGGAAATGCGTTGTGTACAGTAAAGTGTGATGAGAAAGTAA
AGGTGTTTTCTGTTCGAGGAACATCTTTTGAGGCTGCAGCAACAAGTGGAGGTAGTGCTAGTTCAGAAAA
GGCACCAAGTTCTTCATCAGTGGGAATATCAGAGTGGCTTGACCAGAAATTGACAAAAAGTGACCGACCA
GAACTAACTGGTGCCAAAGTGGTGGTATCTGGTGGTCGGGGTTTGAAGAGTGGAGAGAACTTTAAACTGC
TGTATGACCTGGCAGATCAACTACATGCTGCAGTTGGTGCTTCCCGAGCTGCTGTTGATGCTGGCTTTGT
TCCCAATGACATGCAAGTTGGTCAGACAGGAAAAATAGTAGCTCCAGAACTTTATATTGCTGTTGGAATA
TCTGGAGCCATTCAACATTTAACTGGGATGAAGGACAGCAAGACAATCGTGGCTATTAACAAAGATCCAG
AAGCTCCAATTTTCCAGGTGGCAGATTATGGAATAGTTGCAGATTTATTTAAGGTGGTTCCTGAAATGAC
AGAAATACTGAAGAAAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204913 protein sequence
Red=Cloning site Green=Tags(s)

MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKCDKVVQDLCKV
AGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLLPRVAAKLNVAPVSDIIEIKS
PDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGSASSEKAPSSSSVGISEWLDQKLTKSDRP
ELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGI
SGAIQHLTGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145615
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145615.1
RefSeq Size 1464 bp
RefSeq ORF 1002 bp
Locus ID 110842
UniProt ID Q99LC5
Cytogenetics 9 B
MW 35 kDa
Summary Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.UniProtKB/Swiss-Prot Function
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"NM_145615" in other vectors (4)
SKU Description Size Price
MC212235 Etfa (untagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG204913 Etfa (tGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$657.00
MR204913L3 Lenti ORF clone of Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR204913L4 Lenti ORF clone of Etfa (mGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR204913

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204913
Circular map for MR204913

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