Etfa (NM_145615) Mouse Untagged Clone

SKU
MC212235
Etfa (untagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, (10ug)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Etfa
Synonyms 2010200I21Rik; D9Ertd394e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC212235 representing NM_145615
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGAGCAGCGGCGCCTGGGCAGCTCCGGCGGGCGGCCTCATTGCTCCGTTTTCAGAGTACCTTGG
TAATAGCTGAGCATGCAAATGATTCCCTAGCACCCATTACTCTAAATACTATCACTGCAGCTGGACGTCT
TGGAGGTGAAGTGTCCTGCTTAGTAGCTGGAACCAAGTGTGACAAGGTGGTACAGGATCTATGTAAAGTA
GCTGGCGTAGCAAAGGTTCTGGTGGCTCAGCATGATGCATACAAAGGCCTTCTTCCAGAGGAACTCACAC
CATTGATTTTGGAAACTCAGAAGCAGTTCAGTTACACACACATCTGTGCTGGAGCGTCTGCTTTTGGAAA
GAACCTTCTGCCCAGAGTAGCTGCCAAACTTAATGTTGCTCCAGTTTCTGACATCATTGAGATCAAGTCA
CCTGACACATTTGTGAGAACTATCTATGCAGGAAATGCGTTGTGTACAGTAAAGTGTGATGAGAAAGTAA
AGGTGTTTTCTGTTCGAGGAACATCTTTTGAGGCTGCAGCAACAAGTGGAGGTAGTGCTAGTTCAGAAAA
GGCACCAAGTTCTTCATCAGTGGGAATATCAGAGTGGCTTGACCAGAAATTGACAAAAAGTGACCGACCA
GAACTAACTGGTGCCAAAGTGGTGGTATCTGGTGGTCGGGGTTTGAAGAGTGGAGAGAACTTTAAACTGC
TGTATGACCTGGCAGATCAACTACATGCTGCAGTTGGTGCTTCCCGAGCTGCTGTTGATGCTGGCTTTGT
TCCCAATGACATGCAAGTTGGTCAGACAGGAAAAATAGTAGCTCCAGAACTTTATATTGCTGTAGGAATA
TCTGGAGCCATTCAACATTTAGCTGGGATGAAGGACAGCAAGACAATCGTGGCTATTAACAAAGATCCAG
AAGCTCCAATTTTCCAGGTGGCAGATTATGGAATAGTTGCAGATTTATTTAAGGTGGTTCCTGAAATGAC
AGAAATACTGAAGAAAAAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_145615
Insert Size 1002 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145615.4, NP_663590.3
RefSeq Size 1464 bp
RefSeq ORF 1002 bp
Locus ID 110842
UniProt ID Q99LC5
Cytogenetics 9 B
Summary Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145615" in other vectors (4)
SKU Description Size Price
MG204913 Etfa (tGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$657.00
MR204913 Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $457.00
MR204913L3 Lenti ORF clone of Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR204913L4 Lenti ORF clone of Etfa (mGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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