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Eif2s2 (NM_026030) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204882
Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif2s2
Synonyms 38kDa; 2810026E11Rik; AA408636; AA571381; AA986487; AW822225; D2Ertd303e; EIF2; EIF2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204882 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGGGACGAGATGATTTTTGATCCTACTATGAGCAAAAAGAAAAAGAAGAAGAAGAAGCCTTTTA
TGTTAGATGAAGAAGGTGATGCCCAGACAGAAGAAACCCAGCCCTCAGAGACAAAAGAAGTGGAGCCAGA
ACCAACTGAAGAAAAAGACGTGGACGCTGATGAGGAAGACAGTAGGAAGAAAGATGCTTCTGATGACTTA
GATGATTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAGACAAAAAAGATATTTGACATTGATGAAG
CTGAAGAAGCTATAAAGGATGTTAAGATTGAGAGTGATGCTCAAGAGCCAGCCGAGCCAGAGGATGACCT
TGACATTATGCTTGGCAACAAAAAAAAGAAAAAGAAGAATGTTAAATTCCCAGAAGAAGATGAAATACTA
GAAAAAGACGAAGCTTTAGAAGATGAAGACAGCAAAAAAGATGATGGCATTTCATTCAGTAGCCAAACTG
CTTGGGCAGGCTCAGAAAGAGACTACACATATGAGGAGTTGCTGAACCGAGTGTTCAACATCATGAGAGA
AAAGAATCCAGATATGGTTGCTGGAGAGAAGAGGAAATTTGTTATGAAACCTCCACAGGTCGTCCGAGTA
GGAACCAAGAAAACTTCTTTTGTCAATTTTACAGATATCTGTAAACTATTACATCGTCAACCCAAACATC
TCCTTGCATTTTTATTGGCAGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAA
AGGAAGATTCCAACAGAAACAAATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACTTGTCAC
ACATGCCGGTCACCGGACACAATCCTACAGAAGGACACCCGACTCTATTTCTTACAATGTGAAACTTGTC
ATTCTCGATGCTCTGTTGCCAGTATCAAAACTGGCTTCCAGGCTGTCACAGGCAAGCGAGCACAGCTCCG
TGCCAAAGCTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204882 protein sequence
Red=Cloning site Green=Tags(s)

MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDAQTEETQPSETKEVEPEPTEEKDVDADEEDSRKKDASDDL
DDLNFFNQKKKKKKTKKIFDIDEAEEAIKDVKIESDAQEPAEPEDDLDIMLGNKKKKKKNVKFPEEDEIL
EKDEALEDEDSKKDDGISFSSQTAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRV
GTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCH
TCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026030
ORF Size 993 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026030.2, NP_080306.1
RefSeq Size 2513 bp
RefSeq ORF 996 bp
Locus ID 67204
UniProt ID Q99L45
Cytogenetics 2 76.89 cM
MW 38.1 kDa
Summary eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_026030" in other vectors (4)
SKU Description Size Price
MC208067 Eif2s2 (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2), (10ug) 10 ug
$330.00
MG204882 Eif2s2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$500.00
MR204882L3 Lenti ORF clone of Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$600.00
MR204882L4 Lenti ORF clone of Eif2s2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$600.00

Plasmid Map for MR204882

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR204882
Circular map for MR204882

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