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Eif2s2 (NM_026030) Mouse Untagged Clone

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SKU
MC208067
Eif2s2 (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Eif2s2
Synonyms 38kDa; 2810026E11Rik; AA408636; AA571381; AA986487; AW822225; D2Ertd303e; EIF2; EIF2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208067 representing NM_026030
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGGGACGAGATGATTTTTGATCCTACTATGAGCAAAAAGAAAAAGAAGAAGAAGAAGCCTTTTA
TGTTAGATGAAGAAGGTGATGCCCAGACAGAAGAAACCCAGCCCTCAGAGACAAAAGAAGTGGAGCCAGA
ACCAACTGAAGAAAAAGACGTGGACGCTGATGAGGAAGACAGTAGGAAGAAAGATGCTTCTGATGACTTA
GATGATTTGAACTTCTTTAATCAAAAGAAAAAGAAGAAAAAGACAAAAAAGATATTTGACATTGATGAAG
CTGAAGAAGCTATAAAGGATGTTAAGATTGAGAGTGATGCTCAAGAGCCAGCCGAGCCAGAGGATGACCT
TGACATTATGCTTGGCAACAAAAAAAAGAAAAAGAAGAATGTTAAATTCCCAGAAGAAGATGAAATACTA
GAAAAAGACGAAGCTTTAGAAGATGAAGACAGCAAAAAAGATGATGGCATTTCATTCAGTAGCCAAACTG
CTTGGGCAGGCTCAGAAAGAGACTACACATATGAGGAGTTGCTGAACCGAGTGTTCAACATCATGAGAGA
AAAGAATCCAGATATGGTTGCTGGAGAGAAGAGGAAATTTGTTATGAAACCTCCACAGGTCGTCCGAGTA
GGAACCAAGAAAACTTCTTTTGTCAATTTTACAGATATCTGTAAACTATTACATCGTCAACCCAAACATC
TCCTTGCATTTTTATTGGCAGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAA
AGGAAGATTCCAACAGAAACAAATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACTTGTCAC
ACATGCCGGTCACCGGACACAATCCTACAGAAGGACACCCGACTCTATTTCTTACAATGTGAAACTTGTC
ATTCTCGATGCTCTGTTGCCAGTATCAAAACTGGCTTCCAGGCTGTCACAGGCAAGCGAGCACAGCTCCG
TGCCAAAGCTAACTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_026030
Insert Size 996 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026030.2, NP_080306.1
RefSeq Size 2513 bp
RefSeq ORF 996 bp
Locus ID 67204
UniProt ID Q99L45
Cytogenetics 2 76.89 cM
Summary eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026030" in other vectors (4)
SKU Description Size Price
MG204882 Eif2s2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$500.00
MR204882 Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$289.00 $300.00
MR204882L3 Lenti ORF clone of Eif2s2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$600.00
MR204882L4 Lenti ORF clone of Eif2s2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 2 (beta) (Eif2s2) 10 ug
$600.00

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