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Xrcc4 (NM_028012) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR204755
Xrcc4 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Xrcc4
Synonyms 2310057B22Rik; AW413319; AW545101
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR204755 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGGAAAGTAAGCAGAATCTACCTTGCTTCTGAACCCAACGTACCTTATTTTCTGCAAGTGTCTT
GGGAGAGAGCAATAGGATCCGGCTTTGTTATTACACTTACTGACGGCCATTCAGCCTGGACTGCAACAGT
TTCTGAATTGGAGATTTCCCAAGAAGCTGATGACATGGCTATGGAGAAAGGAAAATACATTGATGAGCTG
AGAAAGGCACTGGTGCCAGGGTCAGGAGCAGCTGGTACATACAAGTTCCTCTTTTCTAAGGAGTCTCAGC
ATTTCTCCCTTGAGAAAGAACTGAAAGATGTTTCATTCAGACTTGGTTCCTTCAACCTAGACAAAGTTTC
AAACTCAGCTGAAGTCATAAGAGAACTCATTTGTTACTGCCTGGACACCATTACAGAAAAGCAAGCCAAA
AACGAGCACCTGCAGAAAGAAAATGAAAGGCTCCTGAGAGACTGGAATGATGTTCAAGGCCGATTTGAGA
AATGTGTGAGTGCCAAAGAAGCCTTGGAGGCTGATTTGTATCAAAGATTTATCTTGGTGCTGAATGAGAA
GAAAACAAAGATCCGGAGCTTGCATAAATTGCTAAATGAAGTCCAGCAGCTGGAGGAGAGTACCAAACCT
GAAAGGGAAAATCCGTGTTCTGACAAAACACCTGAGGAGCATGGGCTTTATGATGGAAGCACCGATGAAG
AAAGTGGAGCCCCTGTGCAGGCTGCAGAAACTCTTCATAAGGATGATTCCATATTTTCAAGTCCGGATGT
CACTGACATTGCACCAAGTAGGAAGAGGAGACACCGAATGCAGAAGAATCTTGGGACAGAACCTAAAATG
GCTCCACAGGAGTTGCCGCTACAGGAGAAGGAAAGGCTTGCCTCTTCACTACCCCAGACCTTGAAAGAGG
AGAGCACCTCTGCTGAAAACATGTCTTTAGAAACCCTGAGAAACAGCAGCCCAGAAGATCTCTTTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR204755 protein sequence
Red=Cloning site Green=Tags(s)

MERKVSRIYLASEPNVPYFLQVSWERAIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDEL
RKALVPGSGAAGTYKFLFSKESQHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRELICYCLDTITEKQAK
NEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKP
ERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKM
APQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028012
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028012.1, NM_028012.2, NM_028012.3, NM_028012.4, NP_082288.1
RefSeq Size 1557 bp
RefSeq ORF 981 bp
Locus ID 108138
UniProt ID Q924T3
Cytogenetics 13 C3
MW 37.1 kDa
Summary Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028012" in other vectors (4)
SKU Description Size Price
MC201735 Xrcc4 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), (10ug) 10 ug
$300.00
MG204755 Xrcc4 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$500.00
MR204755L3 Lenti ORF clone of Xrcc4 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$600.00
MR204755L4 Lenti ORF clone of Xrcc4 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$600.00

Plasmid Map for MR204755

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR204755
Circular map for MR204755

file-alt Citations

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