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Xrcc4 (NM_028012) Mouse Untagged Clone

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SKU
MC201735
Xrcc4 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Xrcc4
Synonyms 2310057B22Rik; AW413319; AW545101
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC025538 sequence for NM_028012
CCACGCGTCCGCGGACGCGTGGGATTTTTAGAAGACCGGAAGTGGAGTACTGAGAGGTAGCCAGAAATGG AAAGGAAAGTAAGCAGAATCTACCTTGCTTCTGAACCCAACGTACCTTATTTTCTGCAAGTGTCTTGGGA GAGAGCAATAGGATCCGGCTTTGTTATTACACTTACTGACGGCCATTCAGCCTGGACTGCAACAGTTTCT GAATTGGAGATTTCCCAAGAAGCTGATGACATGGCTATGGAGAAAGGAAAATACATTGATGAGCTGAGAA AGGCACTGGTGCCAGGGTCAGGAGCAGCTGGTACATACAAGTTCCTCTTTTCTAAGGAGTCTCAGCATTT CTCCCTTGAGAAAGAACTGAAAGATGTTTCATTCAGACTTGGTTCCTTCAACCTAGACAAAGTTTCAAAC TCAGCTGAAGTCATAAGAGAACTCATTTGTTACTGCCTGGACACCATTACAGAAAAGCAAGCCAAAAACG AGCACCTGCAGAAAGAAAATGAAAGGCTCCTGAGAGACTGGAATGATGTTCAAGGCCGATTTGAGAAATG TGTGAGTGCCAAAGAAGCCTTGGAGGCTGATTTGTATCAAAGATTTATCTTGGTGCTGAATGAGAAGAAA ACAAAGATCCGGAGCTTGCATAAATTGCTAAATGAAGTCCAGCAGCTGGAGGAGAGTACCAAACCTGAAA GGGAAAATCCGTGTTCTGACAAAACACCTGAGGAGCATGGGCTTTATGATGGAAGCACCGATGAAGAAAG TGGAGCCCCTGTGCAGGCTGCAGAAACTCTTCATAAGGATGATTCCATATTTTCAAGTCCGGATGTCACT GACATTGCACCAAGTAGGAAGAGGAGACACCGAATGCAGAAGAATCTTGGGACAGAACCTAAAATGGCTC CACAGGAGTTGCCGCTACAGGAGAAGGAAAGGCTTGCCTCTTCACTACCCCAGACCTTGAAAGAGGAGAG CACCTCTGCTGAAAACATGTCTTTAGAAACCCTGAGAAACAGCAGCCCAGAAGATCTCTTTGATTAGATA TAAACCCTAGAAAACAGCACTGGTGTTCAATGAACTATATTCTTTATCCAGTTCCTTTGGATGAGAAGAC AGGCTTTCAACTTAGCAGCCATGGTTACTATGTCTTGCAACTTTACTATATGTACCCAATGAATCATACT AGTATGTGACAGTCAGATGGAATGTCAGAGGGACACTTTTCTCCCTTTCTTAATTAAATCTGAGTTTTGT TGCAAAAGGCACATATATCATATTTGTTTATACATTTTTGTAACTTTCTAATTGACTCTTGGAAGATTTT GGAAATAGAACAAAATATCTTTAACACTTTTTTAGTTGCACTTTAAATTATCTTTGTTGAAGCATTTAAT TTATGATGTGACACATATAAAATACATCTTTATCTTGTGTGTAAACAGTCTGAAATCTGATAGTTAATAA AAGTTTGTGTTCTCAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_028012
Insert Size 981 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC025538, AAH25538
RefSeq Size 1502 bp
RefSeq ORF 981 bp
Locus ID 108138
UniProt ID Q924T3
Cytogenetics 13 C3
Summary Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028012" in other vectors (4)
SKU Description Size Price
MG204755 Xrcc4 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$500.00
MR204755 Xrcc4 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$289.00 $300.00
MR204755L3 Lenti ORF clone of Xrcc4 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$600.00
MR204755L4 Lenti ORF clone of Xrcc4 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4) 10 ug
$600.00

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