Mafb (NM_010658) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR204681
Mafb (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb)
$289.00 $330.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mafb
Synonyms kr; Kreisler; Krml; Krml1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_010658
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010658.3, NP_034788.1
RefSeq Size 3389 bp
RefSeq ORF 972 bp
Locus ID 16658
UniProt ID P54841
Cytogenetics 2 80.92 cM
MW 35.8 kDa
Summary Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.UniProtKB/Swiss-Prot Function
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"NM_010658" in other vectors (4)
SKU Description Size Price
MC203214 Mafb (untagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), (10ug) 10 ug
$300.00
MG204681 Mafb (tGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb) 10 ug
$489.00 $530.00
MR204681L3 Lenti ORF clone of Mafb (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb) 10 ug
$630.00
MR204681L4 Lenti ORF clone of Mafb (mGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb) 10 ug
$630.00

Plasmid Map for MR204681

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Cloning scheme for MR204681

Circular map for MR204681

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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