Sox2 (NM_011443) Mouse Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sox2 |
| Synonyms | lc; lcc; Sox; Sox-2; ys; ysb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011443 |
| ORF Size | 957 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011443.4 |
| RefSeq Size | 2457 bp |
| RefSeq ORF | 960 bp |
| Locus ID | 20674 |
| UniProt ID | P48432 |
| Cytogenetics | 3 16.93 cM |
| MW | 34.4 kDa |
| Summary | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in a similar gene in human have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (Sox2ot). provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011443" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC207403 | Sox2 (untagged) - Mouse SRY-box containing gene 2 (Sox2), (10ug) | 10 ug |
$450.00
|
|
| MG204615 | Sox2 (tGFP-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$650.00
|
|
| MR204615L3 | Lenti ORF clone of Sox2 (Myc-DDK-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$750.00
|
|
| MR204615L4 | Lenti ORF clone of Sox2 (mGFP-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$750.00
|
|
Citations
Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells
Lee, Song, Gug et al
Stem Cells International (2022) 2022, 1092184 DOI: 10.1155/2022/1092184
Lee, Song, Gug et al
Stem Cells International (2022) 2022, 1092184 DOI: 10.1155/2022/1092184
Cited by 11
AI Summary: In this study, the Sox2 expression plasmid from OriGene (catalog number MR204615) was used to investigate the regulatory role of NFI-C in stem cell gene expression and proliferation during mineralized tissue development.
Snippet: rowth factor (EGF;20 ng/ml, R&D Systems, Minneapolis, MN). The expression plasmids for Nfic and siNfic were prepared as described previously [7]. The expression plasmid for Sox2 was purchased from OriGene (MR204615) (OriGene, Rockville, MD), and each of those expression plasmids (2 μg) was transiently transfected into ALCs using the Lipofectamine Plus reagent (Invitrogen, Carlsbad, CA) ac
Showing 1-1 of 1 papers.
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