Sox2 (NM_011443) Mouse Tagged ORF Clone
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sox2 |
| Synonyms | lc; lcc; Sox; Sox-2; ys; ysb |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011443 |
| ORF Size | 957 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011443.4 |
| RefSeq Size | 2457 bp |
| RefSeq ORF | 960 bp |
| Locus ID | 20674 |
| UniProt ID | P48432 |
| Cytogenetics | 3 16.93 cM |
| Summary | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in a similar gene in human have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (Sox2ot). provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011443" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC207403 | Sox2 (untagged) - Mouse SRY-box containing gene 2 (Sox2), (10ug) | 10 ug |
$450.00
|
|
| MR204615 | Sox2 (Myc-DDK-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$450.00
|
|
| MR204615L3 | Lenti ORF clone of Sox2 (Myc-DDK-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$750.00
|
|
| MR204615L4 | Lenti ORF clone of Sox2 (mGFP-tagged) - Mouse SRY-box containing gene 2 (Sox2) | 10 ug |
$750.00
|
|
Citations
Low Levels of Sox2 are required for Melanoma Tumor-Repopulating Cell Dormancy
Jia, Yang, Huang et al
Theranostics (2019) 9 (2), 424-435 DOI: 10.7150/thno.29698
Jia, Yang, Huang et al
Theranostics (2019) 9 (2), 424-435 DOI: 10.7150/thno.29698
AI Summary: The study used Sox2 cDNA (OriGene, MG204615) to manipulate Sox2 expression levels in melanoma tumor-repopulating cells, enabling investigation of Sox2's role in regulating tumor cell dormancy and apoptosis.
Snippet: … Sox2 shRNAs and scramble shRNA sequences are shown in Table S2. The Sox2 cDNA was obtained from OriGene (MG204615, Rockville, MD, USA). … Sox2;Cells were transfected with gRNA-Cas9, shRNA, or complementary DNA using Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol. Sox2 shRNAs and scramble shRNA sequences are shown in Table S2. The Sox2 cDNA was obtained from OriGene (MG204615, Rockville, MD, USA)
Matrix softness regulates plasticity of tumour-repopulating cells via H3K9 demethylation and Sox2 expression
Tan, Tajik, Chen et al
Nat Commun (2014) 5, 4619 DOI: 10.1038/ncomms5619
Tan, Tajik, Chen et al
Nat Commun (2014) 5, 4619 DOI: 10.1038/ncomms5619
Cited by 205
AI Summary: OriGene products, specifically Sox2 shRNAs (TG515613) and Sox2 cDNA (MG204615), were used to manipulate Sox2 expression and investigate its role in regulating self-renewal and plasticity of tumour-repopulating cells in this study.
Snippet: Sox2 shRNAs were obtained from Origene (TG515613). The construct sequence is 5′-GCACTACCAGAGCTAACTCAGATAGTACT-3′ for scrambled shRNA, 5′-GTATAACATGATGGAGACGGAGCTGAAGC-3′ for shRNA 1, 5′-AGACGCTCATGAAGAAGGATAAGTACACG-3′ for shRNA 2, 5′-AGCTACGCGCACATGAACGGCTGGAGCAA-3′ for shRNA 3, and 5′-AACATGATGGAGACGGAGCTGAAGCCGCC-3′ for shRNA 4. The Sox2 cDNA was obtained from Origene (MG204615)
Showing 1-2 of 2 papers.
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