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Atg10 (BC023455) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202364
Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atg10
Synonyms Agp10, APG10, Apg10p, Atg10l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202364 representing BC023455
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGATGAGTTCTTTGGAGAAAAAAGCTTCCAGCATTATTGTGCAGAATTCATCAGACATTCACAGC
AGATAGGCGATGGCTGGGAATGGAGAACAGCCAAGGAATGTTCTGATGGCTACATGTGCAAAACACAGTT
TCGAATAAAGAATGAGGCCTCGACACCACATGTGGGAACACCTGCCAGTGTGCTCACATGTCTCCCTACA
GAGGAGAATTTAGAGCTACCAATGGATGATTCTGAAGTGACGAGACCTGCAGCGGTGGCAGAAGTGATTA
AACATGAGTATCATGTCTTATACTCTTGTAGTTACCAAGTGCCGGTTCTTTACTTTAGGGCAAGCTTTTT
AGATGGGAGACCGTTAGCTCTGGAGGATATATGGGAAGGTGTTCATGAGTGCTATAAGCCGCGGCTGCTC
CAGGGACCGTGGGATACCATCACCCAGCAGGAACATCCAATACTTGGGCAACCATTTTTTGTTCTACATC
CCTGCAAGACAAATGAATTCATGACTGCTGTGCTAAAGAATTCACAGAAAATCAATAGAAATGTCAACTA
TATCACATCATGGCTGAGCCTCGTGGGGCCAGTGGTTGGTCTGAATTTACCTCTGAGCTATGCTAAAGCA
ACATCACAATCGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202364 representing BC023455
Red=Cloning site Green=Tags(s)

MEDEFFGEKSFQHYCAEFIRHSQQIGDGWEWRTAKECSDGYMCKTQFRIKNEASTPHVGTPASVLTCLPT
EENLELPMDDSEVTRPAAVAEVIKHEYHVLYSCSYQVPVLYFRASFLDGRPLALEDIWEGVHECYKPRLL
QGPWDTITQQEHPILGQPFFVLHPCKTNEFMTAVLKNSQKINRNVNYITSWLSLVGPVVGLNLPLSYAKA
TSQSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023455
ORF Size 645 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023455, AAH23455
RefSeq Size 1153 bp
RefSeq ORF 647 bp
Locus ID 66795
Cytogenetics 13 C3
MW 42.2 kDa
Summary E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.UniProtKB/Swiss-Prot Function
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"BC023455" in other vectors (4)
SKU Description Size Price
MC206905 Atg10 (untagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268), (10ug) 10 ug
$330.00
MG202364 Atg10 (tGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) 10 ug
$500.00
MR202364L3 Lenti ORF clone of Atg10 (Myc-DDK-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) 10 ug
$600.00
MR202364L4 Lenti ORF clone of Atg10 (mGFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268) 10 ug
$600.00

Plasmid Map for MR202364

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR202364
Circular map for MR202364

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