Coprs (NM_025556) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201479
Coprs (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410022L05 gene (2410022L05Rik)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Coprs
Synonyms 1700029I03Rik; 2410022L05Rik; AA409325; AI256813; C85432; Copr5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201479 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCTCAGGCAGCCACCGGTCGGGGCCCGGGCGAGCGGAGTTCGCAGGAGGCCCCCAGCGCGGAGG
CTGGCTTTGCCACAGCTGACCTCTCTGGTCGGGAGACGGAGACTGAGCTGGCCGTGGATCGACTAGCCAG
TGGAGCTCAGAGCATCCCTGCTGACATTCCTGCCCATGCCGAGGGCCCAAGTTCTGAGGAGGAAGGCTTT
GCAGTGGAGAAGGAAGCTGATGGGGAGTTGTATGCCTGGGAGCTGTCAGAGGGTCCATCCTGCCCACCCA
TGGAACAGGCTGCAGATCTTTTTAATGAGGACTGGGACTTGGAGCTGAAAGCAGACCAAGGAAATCCTTA
CGATGCTGATGACATCCAGGGGAGCATTTCCCAAGAGATCAAGCCTTGGGTGTGCTGTGCACCACAGGGA
GACATGATATACGACCCCAGTTGGCACCATCCACCTCCACTCATACCACACTACTCCAAGATGGTCTTCG
AAACAGGACAGTTTGATGATGCCGAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201479 protein sequence
Red=Cloning site Green=Tags(s)

MDPQAATGRGPGERSSQEAPSAEAGFATADLSGRETETELAVDRLASGAQSIPADIPAHAEGPSSEEEGF
AVEKEADGELYAWELSEGPSCPPMEQAADLFNEDWDLELKADQGNPYDADDIQGSISQEIKPWVCCAPQG
DMIYDPSWHHPPPLIPHYSKMVFETGQFDDAED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025556
ORF Size 519 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025556.3
RefSeq Size 802 bp
RefSeq ORF 522 bp
Locus ID 66423
UniProt ID Q9CQ13
Cytogenetics 8 A1.1
MW 18.7 kDa
Summary Histone-binding protein required for histone H4 methyltransferase activity of PRMT5. Specifically required for histone H4 'Arg-3' methylation mediated by PRMT5, but not histone H3 'Arg-8' methylation, suggesting that it modulates the substrate specificity of PRMT5. Specifically interacts with the N-terminus of histone H4 but not with histone H3, suggesting that it acts by promoting the association between histone H4 and PRMT5. Involved in CCNE1 promoter repression (By similarity). Plays a role in muscle cell differentiation by modulating the recruitment of PRMT5 to the promoter of genes involved in the coordination between cell cycle exit and muscle differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025556" in other vectors (4)
SKU Description Size Price
MC203166 Coprs (untagged) - Mouse RIKEN cDNA 2410022L05 gene (2410022L05Rik), (10ug) 10 ug
$300.00
MG201479 Coprs (tGFP-tagged) - Mouse RIKEN cDNA 2410022L05 gene (2410022L05Rik) 10 ug
$500.00
MR201479L3 Lenti ORF clone of Coprs (Myc-DDK-tagged) - Mouse RIKEN cDNA 2410022L05 gene (2410022L05Rik) 10 ug
$600.00
MR201479L4 Lenti ORF clone of Coprs (mGFP-tagged) - Mouse RIKEN cDNA 2410022L05 gene (2410022L05Rik) 10 ug
$600.00

Plasmid Map for MR201479

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Cloning scheme for MR201479

Circular map for MR201479

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