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Naa50 (NM_028108) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201391
Naa50 (Myc-DDK-tagged) - Mouse N(alpha)-acetyltransferase 50, NatE catalytic subunit (Naa50)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Naa50
Synonyms 2600005K24Rik; 2810441M03Rik; AW112078; Mak3; Mak3p; Nat5; Nat13; San
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201391 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGGTAGCCGGATCGAGCTGGGAGATGTGACGCCACACAATATTAAACAGTTGAAGAGACTGAACC
AGGTCATCTTTCCAGTCAGCTATAATGATAAATTCTACAAGGATGTGCTAGAGGTTGGCGAGCTAGCAAA
ACTTGCATATTTCAATGATATAGCTGTAGGTGCAGTGTGCTGCAGGGTGGATCATTCACAGAATCAAAAG
AGACTTTACATCATGACACTAGGATGCCTTGCACCTTACCGAAGACTAGGAATAGGAACTAAAATGTTAA
ATCATGTCCTAAACATCTGTGAGAAGGATGGCACTTTTGACAATATCTATCTGCATGTCCAGATCAGCAA
TGAGTCAGCGATTGACTTTTACCGGAAGTTTGGCTTTGAGATTATCGAGACAAAGAAGAACTACTATAAG
AGGATAGAGCCTGCAGACGCGCATGTGCTTCAGAAAAACCTCAAAGTCCCATCTGGTCAGAATGCAGAGA
CACAGAAGACAGACAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201391 protein sequence
Red=Cloning site Green=Tags(s)

MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQK
RLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYK
RIEPADAHVLQKNLKVPSGQNAETQKTDN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028108
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 4526 bp
RefSeq ORF 507 bp
Locus ID 72117
UniProt ID Q6PGB6
Cytogenetics 16 B4
MW 19.4 kDa
Summary N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine. Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position. Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins. Autoacetylates in vivo. The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo. Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides. Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10.UniProtKB/Swiss-Prot Function
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"NM_028108" in other vectors (4)
SKU Description Size Price
MC207717 Naa50 (untagged) - Mouse N(alpha)-acetyltransferase 50, NatE catalytic subunit (Naa50), (10ug) 10 ug
$330.00
MG201391 Naa50 (tGFP-tagged) - Mouse N-acetyltransferase 13 (Nat13) 10 ug
$500.00
MR201391L3 Lenti ORF clone of Naa50 (Myc-DDK-tagged) - Mouse N(alpha)-acetyltransferase 50, NatE catalytic subunit (Naa50) 10 ug
$600.00
MR201391L4 Lenti ORF clone of Naa50 (mGFP-tagged) - Mouse N(alpha)-acetyltransferase 50, NatE catalytic subunit (Naa50) 10 ug
$600.00

Plasmid Map for MR201391

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201391
Circular map for MR201391

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