Ang (NM_007447) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR200967
Ang (Myc-DDK-tagged) - Mouse angiogenin, ribonuclease, RNase A family, 5 (Ang), transcript variant 1
$289.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ang |
| Synonyms | AI385586; An; Ang1; Rn; Rnase5; Rnase5a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007447 |
| ORF Size | 435 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007447.2, NP_031473.1 |
| RefSeq Size | 1113 bp |
| RefSeq ORF | 438 bp |
| Locus ID | 11727 |
| UniProt ID | P21570 |
| Cytogenetics | 14 26.37 cM |
| MW | 16.2 kDa |
| Summary | This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene. provided by RefSeq, Jun 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007447" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC205895 | Ang (untagged) - Mouse angiogenin, ribonuclease, RNase A family, 5 (Ang), transcript variant 1, (10ug) | 10 ug |
$150.00
|
|
| MG200967 | Ang (tGFP-tagged) - Mouse angiogenin, ribonuclease A family, member 1 (Ang1) | 10 ug |
$489.00
|
|
| MR200967L3 | Lenti ORF clone of Ang (Myc-DDK-tagged) - Mouse angiogenin, ribonuclease, RNase A family, 5 (Ang), transcript variant 1 | 10 ug |
$450.00
|
|
| MR200967L4 | Lenti ORF clone of Ang (mGFP-tagged) - Mouse angiogenin, ribonuclease, RNase A family, 5 (Ang), transcript variant 1 | 10 ug |
$450.00
|
|
Citations
Senescent cells limit p53 activity via multiple mechanisms to remain viable
Sturmlechner, Sine, Jeganathan et al
Nat Commun (2022) 13 (1), 3722 DOI: 10.1038/s41467-022-31239-x
Sturmlechner, Sine, Jeganathan et al
Nat Commun (2022) 13 (1), 3722 DOI: 10.1038/s41467-022-31239-x
Cited by 46
AI Summary: OriGene products Myc-DDK(Flag)-tagged cDNAs for mouse Ang (#MR200967) and Rnase4 (#MR201018) were used for overexpression studies to investigate their roles in promoting the survival of senescent mouse embryonic fibroblasts by suppressing p53-mediated apoptosis.
Snippet: (shScr;Sigma-Aldrich, #SCH202) was used as a negative control. For shRNA target sequences see Supplementary Table 1. For overexpression studies, we cloned Myc-DDK(Flag)-tagged cDNAs for mouse Ang (Origene, #MR200967) and Rnase4 (Origene, #MR201018) into the lentiviral pTSIN PGK-puro2 backbone68,69. Empty pTSIN vector (EV) was used as negative control in overexpression experiments. Lentivirus p
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
