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Atp5k (NM_007507) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200078
Atp5k (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Atp5k or NM_007507"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp5k
Synonyms 2610008D24Rik; Atp5i; Lfm1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200078 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCCCGGTTCAGGTCTCTCCACTCATCAAGTTCGGCCGGTACTCCGCTCTGATCATCGGCATGG
CATACGGCGCCAAGCGCTACAGTTACCTAAAACCCCGGGCAGAGGAGGAGAGGAGAATAGCAGCGGAGGA
AAAGAAGAGACTAGATGAGTTGAAACGGATTGAGAGAGAACTGGCGGAAGCTCAAGATGACAGCATTCTC
AAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200078 protein sequence
Red=Cloning site Green=Tags(s)

MVPPVQVSPLIKFGRYSALIIGMAYGAKRYSYLKPRAEEERRIAAEEKKRLDELKRIERELAEAQDDSIL
K

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007507
ORF Size 213 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007507.3
RefSeq Size 356 bp
RefSeq ORF 216 bp
Locus ID 11958
UniProt ID Q06185
Cytogenetics 5 53.11 cM
MW 8.2 kDa
Summary Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.UniProtKB/Swiss-Prot Function
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"NM_007507" in other vectors (4)
SKU Description Size Price
MC207236 Atp5k (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$165.00
MG200078 Atp5k (tGFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k) 10 ug
$350.00
MR200078L3 Lenti ORF clone of Atp5k (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200078L4 Lenti ORF clone of Atp5k (mGFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein 10 ug
$450.00

Plasmid Map for MR200078

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200078
Circular map for MR200078

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