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Atp5k (NM_007507) Mouse Untagged Clone

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SKU
MC207236
Atp5k (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $165.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Atp5k
Synonyms 2610008D24Rik; Atp5i; Lfm1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207236 representing NM_007507
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCCCGGTTCAGGTCTCTCCACTCATCAAGTTCGGCCGGTACTCCGCTCTGATCATCGGCATGG
CATACGGCGCCAAGCGCTACAGTTACCTAAAACCCCGGGCAGAGGAGGAGAGGAGAATAGCAGCGGAGGA
AAAGAAGAGACTAGATGAGTTGAAACGGATTGAGAGAGAACTGGCGGAAGCTCAAGATGACAGCATTCTC
AAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_007507
Insert Size 216 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007507.2, NP_031533.2
RefSeq Size 356 bp
RefSeq ORF 216 bp
Locus ID 11958
UniProt ID Q06185
Cytogenetics 5 53.11 cM
Summary Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer, protein-coding transcript.
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Documents
Resources
"NM_007507" in other vectors (4)
SKU Description Size Price
MG200078 Atp5k (tGFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k) 10 ug
$350.00
MR200078 Atp5k (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200078L3 Lenti ORF clone of Atp5k (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200078L4 Lenti ORF clone of Atp5k (mGFP-tagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e (Atp5k), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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