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Gata2 (NM_008090) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG226728
Gata2 (tGFP-tagged) - Mouse GATA binding protein 2 (Gata2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gata2
Synonyms Gata-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226728 representing NM_008090
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCGCCTGAGCAGCCTCGCTGGATGGCGCACCCCGCCGTATTGAATGCGCAGCACCCCGACT
CGCACCATCCGGGCCTGGCGCATAACTACATGGAGCCAGCACAGCTGCTGCCTCCCGACGAGGTGGATGT
CTTCTTCAACCATCTCGACTCGCAGGGCAACCCTTACTACGCCAACCCGGCCCACGCGCGCGCGCGCGTT
TCCTACAGCCCGGCGCATGCCCGTCTCACCGGAGGCCAGATGTGCCGACCACACTTGTTGCACAGCCCAG
GCTTGCCGTGGCTGGACGGGGGCAAAGCAGCTCTCTCTGCCGCCGCTGCCCATCACCACAGTCCCTGGAC
CGTCAGCCCGTTCTCCAAGACCCCGCTGCACCCCTCAGCTGCTGGAGCACCCGGAGGGCCTCTGTCTGTT
TACCCAGGGGCTGCGGGTGGGAGCGGGGGAGGCAGTGGGAGCTCCGTGGCCTCCCTCACCCCCACTGCAG
CCCACTCGGGCTCCCATCTCTTCGGCTTCCCACCCACGCCACCCAAAGAAGTGTCTCCAGACCCCAGCAC
AACAGGAGCTGCTTCCCCGGCCTCTTCTTCTGCAGGGGGTAGTGTAGCCCGGGGTGAGGACAAGGATGGC
GTCAAGTACCAAGTGTCACTCTCCGAGAGCATGAAGATGGAAGGCGGCAGTCCCCTGCGCCCGGGCCTAG
CTACCATGGGCACCCAGCCTGCAACACACCACCCGATACCCACCTATCCCTCCTATGTGCCCGCCGCAGC
TCATGACTATGGCAGCAGTCTCTTCCATCCAGGAGGCTTCCTGGGTGGCCCCGCCTCCAGCTTCACCCCT
AAGCAGAGAAGCAAGGCTCGCTCCTGCTCAGAAGGCCGGGAGTGTGTCAACTGTGGTGCCACAGCCACCC
CTCTCTGGCGACGAGATGGCACGGGCCACTACCTGTGCAATGCCTGTGGGCTCTACCACAAGATGAATGG
ACAGAACCGGCCGCTCATCAAGCCCAAGCGGAGGCTGTCTGCTGCCAGAAGAGCGGGCACCTGTTGTGCA
AATTGTCAGACGACAACCACCACCTTATGGCGCCGGAACGCCAACGGGGACCCTGTGTGCAACGCCTGTG
GCCTCTACTACAAGCTGCACAATGTTAACAGGCCACTGACCATGAAGAAGGAAGGGATCCAGACCCGGAA
TCGGAAGATGTCCAGCAAATCCAAGAAGAGCAAGAAAGGGGCTGAATGTTTCGAGGAGCTCTCCAAGTGC
ATGCAAGAGAAGTCACCGCCCTTCAGTGCGGCTGCCCTGGCTGGACACATGGCACCTGTGGGACACCTCC
CACCTTTTAGTCACTCTGGACACATCCTACCCACGCCCACGCCTATCCACCCTTCCTCCAGTCTCTCTTT
TGGCCACCCCCACCCGTCCAGCATGGTGACTGCCATGGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226728 representing NM_008090
Red=Cloning site Green=Tags(s)

MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARV
SYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHSPWTVSPFSKTPLHPSAAGAPGGPLSV
YPGAAGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSVARGEDKDG
VKYQVSLSESMKMEGGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYGSSLFHPGGFLGGPASSFTP
KQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTCCA
NCQTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSSKSKKSKKGAECFEELSKC
MQEKSPPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008090
ORF Size 1440 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008090.5, NP_032116.4
RefSeq Size 3258 bp
RefSeq ORF 1443 bp
Locus ID 14461
UniProt ID O09100
Cytogenetics 6 39.2 cM
Summary Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.UniProtKB/Swiss-Prot Function
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Resources
"NM_008090" in other vectors (4)
SKU Description Size Price
MC216629 Gata2 (untagged) - Mouse GATA binding protein 2 (Gata2), (10ug) 10 ug
$457.00
MR226728 Gata2 (Myc-DDK-tagged) - Mouse GATA binding protein 2 (Gata2) 10 ug
$289.00 $457.00
MR226728L3 Lenti ORF clone of Gata2 (Myc-DDK-tagged) - Mouse GATA binding protein 2 (Gata2) 10 ug
$757.00
MR226728L4 Lenti ORF clone of Gata2 (mGFP-tagged) - Mouse GATA binding protein 2 (Gata2) 10 ug
$757.00

Plasmid Map for MG226728

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG226728
Circular map for MG226728

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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