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Rab5a (NM_025887) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202355
Rab5a (tGFP-tagged) - Mouse RAB5A, member RAS oncogene family (Rab5a)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $500.00
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Other products for "Rab5a or NM_025887"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab5a
Synonyms 2410015H04Rik; AI663973; AU021172; nnyRab5a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202355 representing NM_025887
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAATCGAGGAGCAACAAGACCCAACGGGCCAAATACTGGAAATAAAATATGCCAGTTCAAACTGG
TCCTTCTAGGAGAGTCTGCTGTTGGCAAATCAAGCCTGGTTCTTCGCTTTGTGAAAGGCCAATTTCATGA
ATTTCAAGAGAGTACCATTGGGGCTGCCTTTCTAACCCAAACTGTGTGTCTTGATGACACAACAGTAAAA
TTTGAAATATGGGATACAGCTGGTCAAGAACGGTATCATAGCTTAGCACCAATGTACTACCGAGGAGCAC
AAGCAGCCATAGTTGTGTATGATATCACAAATGAGGAATCCTTTGCGAGAGCAAAAAACTGGGTTAAAGA
ACTTCAAAGGCAAGCAAGTCCTAATATTGTGATAGCTTTGTCAGGAAACAAAGCTGACTTAGCAAATAAA
AGAGCTGTTGACTTCCAGGAAGCACAGTCCTATGCAGATGACAACAGCTTATTATTTATGGAGACATCAG
CTAAGACATCAATGAATGTAAATGAAATATTTATGGCAATAGCTAAAAAGCTGCCAAAGAATGAACCACA
GAATCCTGGTGCAAACTCAGCCAGAGGACGAGGAGTAGACCTTACTGAGCCTGCACAGCCAGCCAGAAGC
CAGTGTTGTAGTAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202355 representing NM_025887
Red=Cloning site Green=Tags(s)

MANRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVK
FEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANK
RAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPAQPARS
QCCSN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025887
ORF Size 645 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025887.4, NP_080163.1
RefSeq Size 2367 bp
RefSeq ORF 648 bp
Locus ID 271457
UniProt ID Q9CQD1
Cytogenetics 17 27.82 cM
Summary The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025887" in other vectors (4)
SKU Description Size Price
MC207948 Rab5a (untagged) - Mouse RAB5A, member RAS oncogene family (Rab5a), (10ug) 10 ug
$330.00
MR202355 Rab5a (Myc-DDK-tagged) - Mouse RAB5A, member RAS oncogene family (Rab5a) 10 ug
$289.00 $300.00
MR202355L3 Lenti ORF clone of Rab5a (Myc-DDK-tagged) - Mouse RAB5A, member RAS oncogene family (Rab5a) 10 ug
$600.00
MR202355L4 Lenti ORF clone of Rab5a (mGFP-tagged) - Mouse RAB5A, member RAS oncogene family (Rab5a) 10 ug
$600.00

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