Doppel (PRND) (NM_012409) Human Tagged ORF Clone
$489.00
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Doppel |
| Synonyms | dJ1068H6.4; DOPPEL; DPL; PrPLP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012409 |
| ORF Size | 528 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012409.4 |
| RefSeq Size | 4047 bp |
| RefSeq ORF | 531 bp |
| Locus ID | 23627 |
| UniProt ID | Q9UKY0 |
| Cytogenetics | 20p13 |
| Protein Families | Druggable Genome, Transmembrane |
| Summary | This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012409" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223341 | PRND (Myc-DDK-tagged)-Human prion protein 2 (dublet) (PRND) | 10 ug |
$289.00
$450.00
|
|
| RC223341L3 | Lenti ORF clone of Human prion protein 2 (dublet) (PRND), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC223341L4 | Lenti ORF clone of Human prion protein 2 (dublet) (PRND), mGFP tagged | 10 ug |
$750.00
|
|
| SC303977 | PRND (untagged)-Human prion protein 2 (dublet) (PRND) | 10 ug |
$450.00
|
|
Citations
Selective Targeting of Tip Endothelial Cells as a Therapeutic Strategy for Tumor Angiogenesis
Kim, Lee, Azam et al
Adv Sci (Weinh) (2026)
Kim, Lee, Azam et al
Adv Sci (Weinh) (2026)
Cited by 3
AI Summary: OriGene’s Doppel cDNA (catalog #RG223341) was used for transient transfection experiments to investigate Doppel’s role in endothelial tip cell function in this study.
Snippet: For transient transfection in HDMEC, cells were plated in 60 mm dishes (0.7 x 105 cells per dish) and transfected at day 1 of culture. Briefly, Doppel cDNA (RG223341, Origene) was re-suspended in 100 µL of RNase-free water. For each transfection, 0.5 µg of Doppel cDNA
Microfluidic captured patient-derived circulating endothelial cells identify novel targets of pulmonary arterial hypertension
Wahab, Keshavarz, Azam et al
Biomaterials (2025) 323, 123429-123429 DOI: 10.1016/j.biomaterials.2025.123429
Wahab, Keshavarz, Azam et al
Biomaterials (2025) 323, 123429-123429 DOI: 10.1016/j.biomaterials.2025.123429
Cited by 4
AI Summary: OriGene’s PRND plasmid cDNA (catalog #RG223341) was used to transfect pulmonary arterial hypertension endothelial cells, enabling the study of PRND’s role as a potential target in pulmonary arterial hypertension.
Snippet: … Briefly, 0.9X105 PAH-ECs (Cell ID: PHBI-ST-026;Table S3) cells were seeded in each well of 6-well plate and cultured until reaching 70 % confluence. The PRND plasmid cDNA (RG223341, OriGene, MD) were used at a final concentration of 1 μg per 6 well in MV2 medium without serum and antibiotics. …
Showing 1-2 of 2 papers.
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