Doppel (PRND) (NM_012409) Human Untagged Clone

SKU SC303977

PRND (untagged)-Human prion protein 2 (dublet) (PRND)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Doppel
• Synonyms: dJ1068H6.4; DOPPEL; DPL; PrPLP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012409 edited; ATGAGGAAGCACCTGAGCTGGTGGTGGCTGGCCACTGTCTGCATGCTGCTCTTCAGCCAC; CTCTCTGCGGTCCAGACGAGGGGCATCAAGCACAGAATCAAGTGGAACCGGAAGGCCCTG; CCCAGCACTGCCCAGATCACTGAGGCCCAGGTGGCTGAGAACCGCCCGGGAGCCTTCATC; AAGCAAGGCCGCAAGCTCGACATTGACTTCGGAGCCGAGGGCAACAGGTACTACGAGGCC; AACTACTGGCAGTTCCCCGATGGCATCCACTACAACGGCTGCTCTGAGGCTAATGTGACC; AAGGAGGCATTTGTCACCGGCTGCATCAATGCCACCCAGGCGGCGAACCAGGGGGAGTTC; CAGAAGCCAGACAACAAGCTCCACCAGCAGGTGCTCTGGCGGCTGGTCCAGGAGCTCTGC; TCCCTCAAGCATTGCGAGTTTTGGTTGGAGAGGGGCGCAGGACTTCGGGTCACCATGCAC; CAGCCAGTGCTCCTCTGCCTTCTGGCTTTGATCTGGCTCATGGTGAAATAA;
• Restriction Sites: Please inquire
• ACCN: NM_012409
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_012409.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012409.2, NP_036541.2
• RefSeq Size: 4047 bp
• RefSeq ORF: 531 bp
• Locus ID: 23627
• UniProt ID: Q9UKY0
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders. [provided by RefSeq, Jul 2008]