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MAFG (NM_002359) Human Tagged ORF Clone

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SKU
RC221486
MAFG (Myc-DDK-tagged)-Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAFG
Synonyms hMAF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221486 representing NM_002359
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGACCCCCAATAAAGGAAACAAGGCCTTGAAGGTGAAGCGGGAGCCGGGTGAGAATGGCACCAGCC
TGACGGATGAGGAGCTGGTGACCATGTCGGTGCGGGAGCTGAACCAGCACCTGCGGGGCCTGTCCAAGGA
GGAGATCGTCCAGCTGAAGCAGCGCCGGCGCACGCTCAAGAACCGCGGCTACGCTGCCAGCTGCCGCGTG
AAGCGGGTGACGCAGAAGGAGGAGCTGGAGAAGCAGAAGGCGGAGCTGCAGCAGGAGGTGGAGAAGCTGG
CCTCAGAGAACGCCAGCATGAAGCTGGAGCTCGACGCGCTGCGCTCCAAGTACGAGGCGCTGCAGACCTT
CGCCCGGACGGTGGCCCGCAGCCCCGTGGCGCCAGCCCGGGGCCCCCTTGCCGCCGGCCTGGGGCCCCTC
GTCCCAGGCAAGGTGGCCGCCACCAGCGTCATCACAATAGTAAAGTCCAAGACGGATGCCCGATCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221486 representing NM_002359
Red=Cloning site Green=Tags(s)

MTTPNKGNKALKVKREPGENGTSLTDEELVTMSVRELNQHLRGLSKEEIVQLKQRRRTLKNRGYAASCRV
KRVTQKEELEKQKAELQQEVEKLASENASMKLELDALRSKYEALQTFARTVARSPVAPARGPLAAGLGPL
VPGKVAATSVITIVKSKTDARS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002359
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002359.4
RefSeq Size 5043 bp
RefSeq ORF 489 bp
Locus ID 4097
UniProt ID O15525, A0A024R8X1
Cytogenetics 17q25.3
Domains BRLZ, bZIP_Maf
Protein Families Druggable Genome, Transcription Factors
MW 17.7 kDa
Summary Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 PubMed 9166829). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).supplied by OMIM, Mar 2010
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"NM_002359" in other vectors (6)
SKU Description Size Price
RC221486L1 Lenti ORF clone of Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC221486L2 Lenti ORF clone of Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1, mGFP tagged 10 ug
$450.00
RC221486L3 Lenti ORF clone of Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC221486L4 Lenti ORF clone of Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1, mGFP tagged 10 ug
$450.00
RG221486 MAFG (tGFP-tagged) - Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1 10 ug
$350.00
SC118695 MAFG (untagged)-Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 1 10 ug
$150.00

Plasmid Map for RC221486

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221486
Circular map for RC221486

file-alt Citations

A MAFG~MITF complex drives melanoma phenotype switching and progression
Vera, Martinez, Soto-Vargas et al
Nat Commun (2026)
AI Summary: OriGene’s Myc-DDK-tagged MAFG ORF clone (Cat. # RC221486) was used to generate a MAFG-expressing construct for functional studies, enabling the authors to investigate MAFG’s role in melanoma phenotype switching and progression.
Snippet: The CMV promoter and puromycin in pLenti -GFP - puro were replaced with the EF1  promoter and blasticidin, respectively, using standard In -Fusion cloning (Takara Bio, Cat. # 638911) to create pLEGB. The Myc -DDK -tagged ORF clone of MAFG (OriGene, Cat. # RC221486) was cloned into pLEGB to replace GFP and create pLEB -MAFG
The small MAF transcription factor MAFG co-opts MITF to promote melanoma progression
Vera, Martinez, Soto-Vargas et al
bioRxiv (2024)
Cited by 3
Snippet: Lenti-GFP-puro were replaced with the EF1α promoter and blasticidin, respectively, using standard In-Fusion cloning (Takara Bio, Cat. # 638911) to create pLEGB. The Myc-DDK-tagged ORF clone of MAFG (OriGene, Cat. # RC221486) was cloned into pLEGB to replace GFP and create pLEB-MAFG. ORF clones of MAFF and MAFK (OriGene, Cat. # RC215609L3 and #RC223543) were used to replace MAFG in pLEB-MAFG to cr
FigureFigureFigure
MAPK-induced miR-29 restrains melanoma progression by targeting MAFG
Vera, Bok, Jasani et al
bioRxiv (2020)
Snippet: … MAPK-induced miR-29 restrains melanoma progression by targeting MAFG Olga Vera1, Ilah Bok1,2, Neel Jasani1,2, Koji Nakamura1, Xiaonan Xu1, Nicol Mecozzi1, Florian A. …The Myc-DDK-tagged ORF clone of MAFG (RC221486, OriGene USA) was a gift from I. …
MAFG is a potential therapeutic target to restore chemosensitivity in cisplatin-resistant cancer cells by increasing reactive oxygen species
Vera-Puente, Rodriguez-Antolin, Salgado-Figueroa et al
Transl Res (2018)
AI Summary: In this study, OriGene's Myc-DDK-tagged ORF clone of MAFG and the negative control pCMV6 were used for transient transfection experiments to investigate the role of MAFG in cisplatin resistance and chemosensitivity in cancer cells.
Snippet: 16 . A Myc-DDK-tagged ORF clone of MAFG the negative control pCMV6 were used for in transient transfection (OriGene, USA) in combination with MAFG-aptamers following the same procedure. Statistical analysis. The data
Regulación epigénetica de la expresión de ARNs no codificantes y su implicación en la respuesta terapéutica en cáncer de pulmón no microcítico y cáncer de ovario
Vera Puente
Thesis (2018)
Snippet: … Overexpression vectors carry the cDNA sequence of the genes MAFG (RC221486, OriGene), ELK-1 (RC208921, OriGene) or ABCA1 (RC221861, OriGene) followed by the coding sequence of an epitope of Myc for the Cterminus of the protein, to be subsequently detected with antibodies for western blot
Showing 1-5 of 5 papers.
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