Tau-441 (2N4R) (NM_005910) Human Tagged ORF Clone
SKU
RC213312
MAPT (Myc-DDK-tagged)-Human microtubule-associated protein tau (MAPT), transcript variant 2
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Tau |
| Synonyms | DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; tau-40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005910 |
| ORF Size | 1323 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005910.5 |
| RefSeq Size | 5731 bp |
| RefSeq ORF | 1326 bp |
| Locus ID | 4137 |
| UniProt ID | P10636, A0A024RA17 |
| Cytogenetics | 17q21.31 |
| Domains | tubulin-binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, MAPK signaling pathway |
| MW | 45.7 kDa |
| Summary | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005910" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213312L1 | Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC213312L2 | Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RC213312L3 | Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC213312L4 | Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RG213312 | MAPT (tGFP-tagged) - Human microtubule-associated protein tau (MAPT), transcript variant 2 | 10 ug |
$489.00
$657.00
|
|
| SC309606 | MAPT (untagged)-Human microtubule-associated protein tau (MAPT), transcript variant 2 | 10 ug |
$686.00
|
|
Citations
Phospho-tau serine-262 and serine-356 as biomarkers of pre-tangle soluble tau assemblies in Alzheimer's disease
Islam, Hill, Abrahamson et al
Nat Med (2025) 31 (2), 574-588 DOI: 10.1038/s41591-024-03400-0
Islam, Hill, Abrahamson et al
Nat Med (2025) 31 (2), 574-588 DOI: 10.1038/s41591-024-03400-0
Cited by 66
AI Summary: OriGene’s full-length tau441 cDNA (cat. no. RC213312) was used as the template for PCR amplification to generate tau fragments for biochemical studies in this paper.
Snippet: 441, respectively) were amplified using PCR with primers representing the 5′ and 3′ sequence of each fragment, respectively, with the complementary DNA for full-length tau441 (cat. no. RC213312, Origene) as the template. The PCR fragments were cloned directly into the pET_SUMO vector (cohort 3: the Shiley-Marcos Alzheimer's Disease Research Center (ADRC), University of California, San Diego (
Truncating tau reveals different pathophysiological actions of oligomers in single neurons
Hill, Karikari, Lantero-Rodriguez et al
Commun Biol (2021) 4 (1), 1265 DOI: 10.1038/s42003-021-02791-x
Hill, Karikari, Lantero-Rodriguez et al
Commun Biol (2021) 4 (1), 1265 DOI: 10.1038/s42003-021-02791-x
Cited by 11
AI Summary: OriGene’s Tau cDNA (RC213312) was used as a template to generate truncated tau protein fragments, enabling the study to dissect the distinct effects of tau oligomers on neuronal function.
Snippet: The different fragments were PCR amplified using custom-designed primers representing the 5’ and 3’ sequence, respectively, with Tau cDNA (RC213312, Origene) as a template
Fyn kinase inhibition reduces protein aggregation, increases synapse density and improves memory in transgenic and traumatic Tauopathy
Tang, Fesharaki-Zadeh, Takahashi et al
Acta Neuropathol Commun (2020) 8 (1), 96 DOI: 10.1186/s40478-020-00976-9
Tang, Fesharaki-Zadeh, Takahashi et al
Acta Neuropathol Commun (2020) 8 (1), 96 DOI: 10.1186/s40478-020-00976-9
Cited by 74
AI Summary: OriGene products contributed to this study by providing plasmids expressing human Tau (RC213312) and Fyn (RC224691), which were used for transient transfection to model Tauopathy mechanisms in cell culture experiments.
Snippet: and 1% penicillin/streptomycin (100 U/mL). Cells were plated at 40,000 cells/well onto 8-well chamber slides (Thermo Scientific 154,941). Transient transfection with plasmids expressing human Tau (Origene, RC213312) and Fyn (Origene, RC224691) was performed using Lipofectamine 2000 transfection reagent (Invitrogen). Three hours later, 2 μM AZD0530 in DMSO or DMSO (vehicle) was added to the t
Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells
Chadchankar, Korboukh, Conway et al
PLoS ONE (2019) 14 (11), e0225145 DOI: 10.1371/journal.pone.0225145
Chadchankar, Korboukh, Conway et al
PLoS ONE (2019) 14 (11), e0225145 DOI: 10.1371/journal.pone.0225145
AI Summary: OriGene contributed plasmids for tau (4R/2N), α-synuclein, USP14, and UCHL5, which were used to express these proteins in mammalian cells for investigating the distinct effects of inactive USP14 and UCHL5 on the accumulation of ubiquitinated proteins.
Snippet: … Plasmids for tau (4R/2N), α-synuclein, USP14 and UCHL5 were purchased from Origene. Mutant plasmids USP14 C114A, USP14 WT ΔUBL, USP14 C114A ΔUBL and UCHL5 C88A were generated from the wildtype plasmid by Genscript. …
Identification of a Novel Selective and Potent Inhibitor of Glycogen Synthase Kinase-3
Noori, Bhatt, Courreges et al
Am. J. Physiol., Cell Physiol. (2019)
Noori, Bhatt, Courreges et al
Am. J. Physiol., Cell Physiol. (2019)
BIN1 favors the spreading of Tau via extracellular vesicles
Crotti, Sait, McAvoy et al
Sci Rep (2019) 9 (1), 9477 DOI: 10.1038/s41598-019-45676-0
Crotti, Sait, McAvoy et al
Sci Rep (2019) 9 (1), 9477 DOI: 10.1038/s41598-019-45676-0
AI Summary: OriGene products, specifically the plasmids expressing human Tau 2N4R (Cat#: RC213312) and murine Bin1 transcript variant 2 (Cat#: MR207643), were used to investigate the roles of BIN1 and Tau in extracellular vesicle-mediated Tau spreading in Alzheimer's disease models.
Snippet: pLVX Ef1a Ires puro vector from Clontech was cut with EcoRI/SpeI. Plasmids expressing human Tau 2N4R (Cat#: RC213312) and murine Bin1 transcript variant 2 (Cat#:MR207643) are commercially available (Origene). An insert encoding 2N4R human Tau with a P301L mutation and YFP at the C-terminus was synthesized as a linear DNA string, and cloned into pLVX Ires-Puro lentiviral plasmid from Clontech usin
Showing 1-6 of 9 papers.
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