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LRRC8A (NM_019594) Human Tagged ORF Clone

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SKU
RC208632
LRRC8A (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $742.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC8A
Synonyms AGM5; HsLRRC8A; LRRC8; SWELL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208632 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGT
GGGATGTGTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTCTTCGGGGGGACGCTGCAGGT
CACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTCACCAAGGACTCCTGCAATGATTCGTTCCGG
GGCTGGGCAGCCCCTGGCCCGGAGCCCACCTACCCCAACTCCACCATTCTGCCGACCCCTGACACGGGCC
CCACAGGCATCAAGTATGACCTGGACCGGCACCAGTACAACTACGTGGACGCTGTGTGCTATGAGAACCG
ACTGCACTGGTTTGCCAAGTACTTCCCCTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGC
AACTTCTGGTTCAAATTCCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTGCTGAAGTGCT
TCGACTCGCCCTGGACCACGAGGGCCCTGTCGGAGACAGTGGTGGAGGAGAGCGACCCCAAGCCGGCCTT
CAGCAAGATGAATGGGTCCATGGACAAAAAGTCATCGACCGTCAGTGAGGACGTGGAGGCCACCGTGCCC
ATGCTGCAGCGGACCAAGTCACGGATCGAGCAGGGTATCGTGGACCGCTCAGAGACGGGCGTGCTGGACA
AGAAGGAGGGGGAGCAAGCCAAGGCGCTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGG
GGACATTGTGTACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCATCCTCATCATCTGC
TACACCGTCTACTACGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATTGAGAGCCTGACGG
GCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACACTCTTCAAGATCCTGGCGTCCTTCTACATCAG
CCTAGTCATCTTCTACGGCCTCATCTGCATGTATACACTGTGGTGGATGCTACGGCGCTCCCTCAAGAAG
TACTCGTTTGAGTCGATCCGTGAGGAGAGCAGCTACAGCGACATCCCCGACGTCAAGAACGACTTCGCCT
TCATGCTGCACCTCATTGACCAATACGACCCGCTCTACTCCAAGCGCTTCGCCGTCTTCCTGTCGGAGGT
GAGTGAGAACAAGCTGCGGCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTCCGGCAGCGGCTC
ACCAAGAACGCGCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGTGGCATCCCTGACACTGTGTTTG
ACCTGGTGGAGCTGGAGGTCCTCAAGCTGGAGCTGATCCCCGACGTGACCATCCCGCCCAGCATTGCCCA
GCTCACGGGCCTCAAGGAGCTGTGGCTCTACCACACAGCGGCCAAGATTGAAGCGCCCGCGCTGGCCTTC
CTGCGCGAGAACCTGCGGGCGCTGCACATCAAGTTCACCGACATCAAGGAGATCCCGCTGTGGATCTATA
GCCTGAAGACACTGGAGGAGCTGCACCTGACGGGCAACCTGAGCGCGGAGAACAACCGCTACATCGTCAT
CGACGGGCTGCGGGAGCTCAAACGCCTCAAGGTGCTGCGGCTCAAGAGCAACCTAAGCAAGCTGCCACAG
GTGGTCACAGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATCGTCC
TCAACAGCCTCAAGAAGATGGCGAACCTGACTGAGCTGGAGCTGATCCGCTGTGACCTGGAGCGCATCCC
CCACTCCATCTTCAGCCTCCACAACCTGCAGGAGATTGACCTCAAGGACAACAACCTCAAGACCATCGAG
GAGATCATCAGCTTCCAGCACCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACA
TCCCCATCCAGATCGGCAACCTCACCAACCTGGAGCGCCTCTACCTGAACCGCAACAAGATCGAGAAGAT
CCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC
CCTGCCGACATCGGCCTCCTGCAGAACCTCCAGAACCTAGCCATCACGGCCAACCGGATCGAGACGCTCC
CTCCGGAGCTCTTCCAGTGCCGGAAGCTGCGGGCCCTGCACCTGGGCAACAACGTGCTGCAGTCACTGCC
CTCCAGGGTGGGCGAGCTGACCAACCTGACGCAGATCGAGCTGCGGGGCAACCGGCTGGAGTGCCTGCCT
GTGGAGCTGGGCGAGTGCCCACTGCTCAAGCGCAGCGGCTTGGTGGTGGAGGAGGACCTGTTCAACACAC
TGCCACCCGAGGTGAAGGAGCGGCTGTGGAGGGCTGACAAGGAGCAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208632 protein sequence
Red=Cloning site Green=Tags(s)

MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFR
GWAAPGPEPTYPNSTILPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACS
NFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVP
MLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFILIIC
YTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKK
YSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRL
TKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAF
LRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQ
VVTDVGVHLQKLSINNEGTKLIVLNSLKKMANLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIE
EIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFL
PADIGLLQNLQNLAITANRIETLPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLP
VELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019594
ORF Size 2430 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019594.3
RefSeq Size 4368 bp
RefSeq ORF 2433 bp
Locus ID 56262
UniProt ID Q8IWT6, A0A024R892
Cytogenetics 9q34.11
Protein Families Transmembrane
MW 94.2 kDa
Summary This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_019594" in other vectors (6)
SKU Description Size Price
RC208632L1 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,042.00
RC208632L2 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, mGFP tagged 10 ug
$1,042.00
RC208632L3 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,042.00
RC208632L4 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, mGFP tagged 10 ug
$1,042.00
RG208632 LRRC8A (tGFP-tagged) - Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2 10 ug
$942.00
SC127734 LRRC8A (untagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2 10 ug
$743.00

Plasmid Map for RC208632

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208632
Circular map for RC208632

file-alt Citations

Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels and regulates cGAMP transport
Zheng, Hagino, Wang et al
Mol Cell (2025) 85 (24), 4621-4632.e7 DOI: 10.1016/j.molcel.2025.11.014
Cited by 2
AI Summary: OriGene’s pCMV-SWELL1-Flag plasmid (RC208632) was used to overexpress SWELL1 in HEK293 cells, enabling the study of SWELL1’s interaction with puromycin-sensitive aminopeptidase and its functional role in volume-regulated anion channels.
Snippet: HEK293 cells were transfected with either pCMV vector or pCMV-SWELL1-Flag (OriGene, RC208632) using Lipofectamine 2000 transfection agent (Life Technologies) for 2 days prior to lysis in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl, pH 7.4, 150 mM
Chloride Channel-3 (ClC-3) Modifies the Trafficking of Leucine-Rich Repeat-Containing 8A (LRRC8A) Anion Channels
Stark, Nguyen, Bacon et al
J Membr Biol (2022) 256 (2), 125-135 DOI: 10.1007/s00232-022-00271-9
Cited by 5
AI Summary: In this study, the ClC-3 (clone #RC208632, NM_019594) and Rab7 (NM_004637, clone) plasmids from OriGene were used to investigate how ClC-3 influences the intracellular trafficking of LRRC8A anion channels in HEK293 cells.
Snippet: Chloride channel-3 (ClC-3) Cl − /H + antiporters and leucine-rich repeat-containing 8 (LRRC8) family anion channels have both been associated with volume-regulated Save;clone #RC208632, NM_019594) was obtained from Origene Technologies (Rockville, MD). … Rab7 (NM_004637, clone
Structure of the human volume regulated anion channel
Kefauver, Saotome, Dubin et al
Elife (2018) 7 DOI: 10.7554/eLife.38461
AI Summary: OriGene products contributed to this study by providing the human SWELL1 (LRRC8A) cDNA clone (Cat# RC209074), which was used for protein expression and subsequent structural and functional analyses.
FigureFigureFigure
Structure of the Human Volume Regulated Anion Channel
Kefauver, Saotome, Dubin et al
bioRxiv (2018)
Snippet: Protein expression and purification Human LRRC8A (Origene #RC208632) was cloned with a C-terminal FLAG-tag (DYKDDDDK) separated by a triple glycine linker (LRRC8A-GGG-FLAG) into a pcDNA3.1/Zeo(-) vector using Gibson cloning View as HTML
Down-regulation of LRRC8A protects Human Ovarian and Alveolar Carcinoma cells against Cisplatin-induced expression of p53, MDM2, p21 and Caspase-9/-3 activation
Sørensen, Nielsen, Thorsteinsdottir et al
Am. J. Physiol., Cell Physiol. (2016)
Cited by 61
AI Summary: In this study, the OriGene human LRRC8A cDNA ORF clone (RC208632) was used to investigate the role of LRRC8A in cisplatin resistance, enabling the authors to examine how altering LRRC8A expression affects apoptosis-related signaling in carcinoma cells.
Snippet: obtained by PCR using AccuPol polymerase (VWR, Denmark), a human LRRC8A cDNA ORF clone (RC208632, OriGene) and the following primers, LRRC8A (forward);5`-ATA TAA GCA GAG CTG GTT TAG TGA ACC GTC AGA TCG GGT TGA ACC ATG ATT CCG ... Cite SaveSaving
Showing 1-5 of 5 papers.
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