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LRRC8A (NM_019594) Human Untagged Clone

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SKU
SC127734
LRRC8A (untagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2
  $743.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LRRC8A
Synonyms AGM5; HsLRRC8A; LRRC8; SWELL1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127734 sequence for NM_019594 edited (data generated by NextGen Sequencing)
ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTG
AAGCCGTGGTGGGATGTGTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTC
TTCGGGGGGACGCTGCAGGTCACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTC
ACCAAGGACTCCTGCAATGATTCGTTCCGGGGCTGGGCAGCCCCTGGCCCGGAGCCCACC
TACCCCAACTCCACCATTCTGCCGACCCCTGACACGGGCCCCACAGGCATCAAGTATGAC
CTGGACCGGCACCAGTACAACTACGTGGACGCTGTGTGCTATGAGAACCGACTGCACTGG
TTTGCCAAGTACTTCCCCTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGC
AACTTCTGGTTCAAATTCCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTG
CTGAAGTGCTTCGACTCGCCCTGGACCACGAGGGCCCTGTCGGAGACAGTGGTGGAGGAG
AGCGACCCCAAGCCGGCCTTCAGCAAGATGAATGGGTCCATGGACAAAAAGTCATCGACC
GTCAGTGAGGACGTGGAGGCCACCGTGCCCATGCTGCAGCGGACCAAGTCACGGATCGAG
CAGGGTATCGTGGACCGCTCAGAGACGGGCGTGCTGGACAAGAAGGAGGGGGAGCAAGCC
AAGGCGCTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGGGGACATTGTG
TACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCATCCTCATCATCTGC
TACACCGTCTACTACGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATTGAG
AGCCTGACGGGCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACACTCTTCAAGATC
CTGGCGTCCTTCTACATCAGCCTAGTCATCTTCTACGGCCTCATCTGCATGTACACACTG
TGGTGGATGCTACGGCGCTCCCTCAAGAAGTACTCGTTTGAGTCGATCCGTGAGGAGAGC
AGCTACAGCGACATCCCCGACGTCAAGAACGACTTCGCCTTCATGCTGCACCTCATTGAC
CAATACGACCCGCTCTACTCCAAGCGCTTCGCCGTCTTCCTGTCGGAGGTGAGTGAGAAC
AAGCTGCGGCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTCCGGCAGCGGCTC
ACCAAGAACGCGCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGTGGCATCCCTGAC
ACTGTGTTTGACCTGGTGGAGCTGGAGGTCCTCAAGCTGGAGCTGATCCCCGACGTGACC
ATCCCGCCCAGCATTGCCCAGCTCACGGGCCTCAAGGAGCTGTGGCTCTACCACACAGCG
GCCAAGATTGAAGCGCCCGCGCTGGCCTTCCTGCGCGAGAACCTGCGGGCGCTGCACATC
AAGTTCACTGACATCAAGGAGATCCCGCTGTGGATCTATAGCCTGAAGACACTGGAGGAG
CTGCACCTGACGGGCAACCTGAGCGCGGAGAACAACCGCTACATCGTCATCGACGGGCTG
CGGGAGCTCAAACGCCTCAAGGTGCTGCGGCTCAAGAGCAACCTAAGCAAGCTGCCACAG
GTGGTCACAGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAG
CTCATCGTCCTCAACAGCCTCAAGAAGATGGCGAACCTGACTGAGCTGGAGCTGATCCGC
TGTGACCTGGAGCGCATCCCCCACTCCATCTTCAGCCTCCACAACCTGCAGGAGATTGAC
CTCAAGGACAACAACCTCAAGACCATCGAGGAGATCATCAGCTTCCAGCACCTGCACCGC
CTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACATCCCCATCCAGATCGGCAAC
CTCACCAACCTGGAGCGCCTCTACCTGAACCGCAACAAGATCGAGAAGATCCCCACCCAG
CTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC
CCTGCCGACATCGGCCTCCTGCAGAACCTCCAGAACCTAGCCATCACGGCCAACCGGATC
GAGACGCTCCCTCCGGAGCTCTTCCAGTGCCGGAAGCTGCGGGCCCTGCACCTGGGCAAC
AACGTGCTGCAGTCACTGCCCTCCAGGGTGGGCGAGCTGACCAACCTGACGCAGATCGAG
CTGCGGGGCAACCGGCTGGAGTGCCTGCCTGTGGAGCTGGGCGAGTGCCCACTGCTCAAG
CGCAGCGGCTTGGTGGTGGAGGAGGACCTGTTCAACACACTGCCACCCGAGGTGAAGGAG
CGGCTGTGGAGGGCTGACAAGGAGCAGGCCTGA

Clone variation with respect to NM_019594.3
1014 t=>c;1509 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_019594 unedited
ACTCACTATAGGGGCGGCCGCGAATCGGTACGAGGCTGCGGCCGGCGGCGGGACGGAGCG
GCCGGGGCCTGGGGCTGCCTGCCGGGCGGCCGGGCGCGGCGAGCCCAGGGAGGCAGCGTC
CATGGAGCAAAAGGAATGCCAGGATCCTGCACAGGCAGACGCGGGCCAGCCTCAGCACCG
ACAGCCGACGCGCAGATAGCAGAGCCATCCTTGGGGTTGAACCATGATTCCGGTGACAGA
GCTCCGCTACTTTGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGTGGGATGT
GTTCACAGACTACATCTCTATCGTCATGCTGATGATTGCCGTCTTCGGGGGGACGCTGCA
GGTCACCCAAGACAAGATGATCTGCCTGCCTTGTAAGTGGGTCACCAAGGACTCCTGCAA
TGATTCGTTCCGGGGCTGGGCAGCCCCTGGCCCGGAGCCCACCTACCCCAACTCCACCAT
TCTGCCGACCCCTGACACGGGCCCCACAGGCATCAAGTATGACCTGGACCGGCACCAGTA
CAACTACGTGGACGCTGTGTGCTATGAGAACCGACTGCACTGGTTTGCCAAGTACTTCCC
CTACCTGGTGCTTCTGCACACGCTCATCTTCCTGGCCTGCAGCAACTTCTGGTTCAAATT
CCCGCGCACCAGCTCGAAGCTGGAGCACTTTGTGTCTATCCTGCTGAAGTGCTTCGACTC
GCCCTGGGACCACGAGGCCCTGTCGGAGACAGTGGTGGNAGAGAGCGACCCCAAGCCGGC
CTTCAGCAGATGAATGGGTCCATGGGACAAAGTCATCGACCGGTCAGTGAGACGTGGAGG
CACCGTNGCCATGCTGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_019594 unedited
TCTATGGACGCGGCCGCTTTCTANAGTCGAGTTTTTTTTTTTTTTTTTTAATACCCAAAC
CATCCATTTTCAAAGCTTTTTTTTTAAAAAAATGTTTTTTGATTAAAAAAAAAAAAACAA
AAAACTAAAACTGCCACAAATACTGAAGAGGCAAGCTCCAGGCCTGGCCTTTCCAGCTGG
AGGCCTGCTCTCCCGTGGACCGCACCGCAGAGCCCAGCCCGGCACTGCTGGGTGACCGTC
CCATGGCTGGTTCGCCCCGGACCACTCTGCTGAAGTCCAGGTGGGTGTTAAATGCCCGGC
GGCATCGCTAAATAAGGACAAGGGGAAAAGGCAGCCCACCTTTCCCTGAACACCTTCCAT
CTGGGCTGAACTTTCTTGGGAATGTATAAGTTATCTGGATGAAGGAGGCGGGAGATGGAG
AAAAATAAATACCAAGAACTAAAGTGAGGGTCCCAGGACCTCTGGTGGCAGCTCAGCCCC
TGGGGCAGAGCTGGCCTCCAGGGAGGAGACCCTGATTATCCAAATACTGCGCTCTTGAGA
CTTGCTCTCCTCCACAAGCACTTGCCCTGGGGGACGTGAGTCTCAGAGGGAGAAAAGGGG
CCAGCCCCACAGATACTGTCCTCTCGCTCTGGCCTGACTCACAAGCGGCCCCAGGCTCCT
GCCCAGCCACGAGCTGTCCTGCCTGTCCGGGAGTTCTGGGAGAACCTAGGCCTGCCCCTC
CGGCCCTGAGGACCTGGCAGCGGTCCTGCTGCTTGCTGTGCTGGGCCCGCCTCGCTCAAC
CCTGCTCCTTTGTAGCCCCTCCAAGCCGGTCTCTCACCTNGGGTGGAAGGGTGTGAACAG
TCCCTCTACACCACAAACCGNTGNGCTTGACCAGGGGNACTCCCCAGCTTCAAGGCAGAA
CTCCACCCGNTGCCCCCACTTAACTGGTCAGGTGCACACC
Restriction Sites NotI-NotI |
ACCN NM_019594
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019594.2, NP_062540.2
RefSeq Size 4296 bp
RefSeq ORF 2433 bp
Locus ID 56262
UniProt ID Q8IWT6
Cytogenetics 9q34.11
Protein Families Transmembrane
Summary This gene encodes a protein belonging to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. This family member is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-3) encode the same protein.
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Documents
Resources
"NM_019594" in other vectors (6)
SKU Description Size Price
RC208632 LRRC8A (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2 10 ug
$742.00
RC208632L1 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,042.00
RC208632L2 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, mGFP tagged 10 ug
$1,042.00
RC208632L3 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,042.00
RC208632L4 Lenti ORF clone of Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2, mGFP tagged 10 ug
$1,042.00
RG208632 LRRC8A (tGFP-tagged) - Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2 10 ug
$942.00

file-alt Citations

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