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IFI16 (NM_005531) Human Tagged ORF Clone

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SKU
RC202193
IFI16 (Myc-DDK-tagged)-Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,001.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IFI16
Synonyms IFNGIP1; PYHIN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202193 representing NM_005531.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGAAAAAAATACAAGAACATTGTTCTACTAAAAGGATTAGAGGTCATCAATGATTATCATTTTAGA
ATGGTTAAGTCCTTACTGAGCAACGATTTAAAACTTAATTTAAAAATGAGAGAAGAGTATGACAAAATT
CAGATTGCTGACTTGATGGAAGAAAAGTTCCGAGGTGATGCTGGTTTGGGCAAACTAATAAAAATTTTC
GAAGATATACCAACGCTTGAAGACCTGGCTGAAACTCTTAAAAAAGAAAAGTTAAAAGTAAAAGGACCA
GCCCTATCAAGAAAGAGGAAGAAGGAAGTGGATGCTACTTCACCTGCACCCTCCACAAGCAGCACTGTC
AAAACTGAAGGAGCAGAGGCAACTCCTGGAGCTCAGAAAAGAAAAAAATCAACCAAAGAAAAGGCTGGA
CCCAAAGGGAGTAAGGTGTCCGAGGAACAGACTCAGCCTCCCTCTCCTGCAGGAGCCGGCATGTCCACA
GCCATGGGCCGTTCCCCATCTCCCAAGACCTCATTGTCAGCTCCACCCAACACTTCTTCAACTGAGAAC
CCGAAAACAGTGGCCAAATGTCAGGTAACTCCCAGAAGAAATGTTCTCCAAAAACGCCCAGTGATAGTG
AAGGTACTGAGTACAACAAAGCCATTTGAATATGAGACCCCAGAAATGGAGAAAAAAATAATGTTTCAT
GCTACAGTGGCTACACAGACACAGTTCTTCCATGTGAAGGTTTTAAACACCAGCTTGAAGGAGAAATTC
AATGGAAAGAAAATCATCATCATATCAGATTATTTGGAATATGATAGTCTCCTAGAGGTCAATGAAGAA
TCTACTGTATCTGAAGCTGGTCCTAACCAAACGTTTGAGGTTCCAAATAAAATCATCAACAGAGCAAAG
GAAACTCTGAAGATTGATATTCTTCACAAACAAGCTTCAGGAAATATTGTATATGGGGTATTTATGCTA
CATAAGAAAACAGTAAATCAGAAGACCACAATCTACGAAATTCAGGATGATAGAGGAAAAATGGATGTA
GTGGGGACAGGACAATGTCACAATATCCCCTGTGAAGAAGGAGATAAGCTCCAACTTTTCTGCTTTCGA
CTTAGAAAAAAGAACCAGATGTCAAAACTGATTTCAGAAATGCATAGTTTTATCCAGATAAAGAAAAAA
ACAAACCCGAGAAACAATGACCCCAAGAGCATGAAGCTACCCCAGGAACAGAGTCAGCTTCCAAATCCT
TCAGAGGCCAGCACAACCTTCCCTGAGAGCCATCTTCGGACTCCTCAGATGCCACCAACAACTCCATCC
AGCAGTTTCTTCACCAAGAAAAGTGAAGACACAATCTCCAAAATGAATGACTTCATGAGGATGCAGATA
CTGAAGGAAGGGAGTCATTTTCCAGGACCGTTCATGACCAGCATAGGCCCAGCTGAGAGCCATCCCCAC
ACTCCTCAGATGCCTCCATCAACACCAAGCAGCAGTTTCTTAACCACGTTGAAACCAAGACTGAAGACT
GAACCTGAAGAAGTTTCCATAGAAGACAGTGCCCAGAGTGACCTCAAAGAAGTGATGGTGCTGAACGCA
ACAGAATCATTTGTATATGAGCCCAAAGAGCAGAAGAAAATGTTTCATGCCACAGTGGCAACTGAGAAT
GAAGTCTTCCGAGTGAAGGTTTTTAATATTGACCTAAAGGAGAAGTTCACCCCAAAGAAGATCATTGCC
ATAGCAAATTATGTTTGCCGCAATGGGTTCCTGGAGGTATATCCTTTCACACTTGTGGCTGATGTGAAT
GCTGACCGAAACATGGAGATCCCAAAAGGATTGATTAGAAGTGCCAGCGTAACTCCTAAAATCAATCAG
CTTTGCTCACAAACTAAAGGAAGTTTTGTGAATGGGGTGTTTGAGGTACATAAGAAAAATGTAAGGGGT
GAATTCACTTATTATGAAATACAAGATAATACAGGGAAGATGGAAGTGGTGGTGCATGGACGACTGACC
ACAATCAACTGTGAGGAAGGAGATAAACTGAAACTCACCTGCTTTGAATTGGCACCGAAAAGTGGGAAT
ACCGGGGAGTTGAGATCTGTAATTCATAGTCACATCAAGGTCATCAAGACCAGGAAAAACAAGAAAGAC
ATACTCAATCCTGATTCAAGTATGGAAACTTCACCAGACTTTTTCTTC
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Protein Sequence
>Peptide sequence encoded by RC202193
Blue=ORF Red=Cloning site Green=Tag(s)

MGKKYKNIVLLKGLEVINDYHFRMVKSLLSNDLKLNLKMREEYDKIQIADLMEEKFRGDAGLGKLIKIF
EDIPTLEDLAETLKKEKLKVKGPALSRKRKKEVDATSPAPSTSSTVKTEGAEATPGAQKRKKSTKEKAG
PKGSKVSEEQTQPPSPAGAGMSTAMGRSPSPKTSLSAPPNTSSTENPKTVAKCQVTPRRNVLQKRPVIV
KVLSTTKPFEYETPEMEKKIMFHATVATQTQFFHVKVLNTSLKEKFNGKKIIIISDYLEYDSLLEVNEE
STVSEAGPNQTFEVPNKIINRAKETLKIDILHKQASGNIVYGVFMLHKKTVNQKTTIYEIQDDRGKMDV
VGTGQCHNIPCEEGDKLQLFCFRLRKKNQMSKLISEMHSFIQIKKKTNPRNNDPKSMKLPQEQSQLPNP
SEASTTFPESHLRTPQMPPTTPSSSFFTKKSEDTISKMNDFMRMQILKEGSHFPGPFMTSIGPAESHPH
TPQMPPSTPSSSFLTTLKPRLKTEPEEVSIEDSAQSDLKEVMVLNATESFVYEPKEQKKMFHATVATEN
EVFRVKVFNIDLKEKFTPKKIIAIANYVCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQ
LCSQTKGSFVNGVFEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGN
TGELRSVIHSHIKVIKTRKNKKDILNPDSSMETSPDFFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using RC202193 also available, TP302193
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005531
ORF Size 2187 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 2709 bp
RefSeq ORF 2190 bp
Locus ID 3428
UniProt ID Q16666
Cytogenetics 1q23.1
Domains HIN, PAAD_DAPIN
Protein Families Transcription Factors
MW 82 kDa
Summary This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2011
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Documents
Resources
"NM_005531" in other vectors (6)
SKU Description Size Price
RC202193L1 Lenti ORF clone of Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2, Myc-DDK-tagged 10 ug
$1,301.00
RC202193L2 Lenti ORF clone of Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2, mGFP tagged 10 ug
$1,301.00
RC202193L3 Lenti ORF clone of Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2, Myc-DDK-tagged 10 ug
$1,301.00
RC202193L4 Lenti ORF clone of Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2, mGFP tagged 10 ug
$1,301.00
RG202193 IFI16 (tGFP-tagged) - Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2 10 ug
$789.00 $1,201.00
SC116712 IFI16 (untagged)-Human interferon, gamma-inducible protein 16 (IFI16), transcript variant 2 10 ug
$1,002.00

Plasmid Map for RC202193

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202193
Circular map for RC202193

file-alt Citations

IFI16 senses and protects stalled replication forks
Gamble, Ward, Wheeler et al
Mol Cell (2026) 86 (2), 258-272.e6 DOI: 10.1016/j.molcel.2025.12.024
Cited by 1
AI Summary: In this study, OriGene's pCMV6-Entry:IFI16-mycDKK plasmid (RC202193) was used to investigate the role of IFI16 in sensing and protecting stalled replication forks.
Snippet: pCMV6-Entry:IFl16-mycDKK Origene RC202193 pFB-2XMBP-HLTF-10Xhis Halder et al.43,47 N/A pFB-2XMBP-IFl16-10Xhis This paper N/A
IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP
Roy, Ghosh, Kumar et al
Elife (2019) 8 DOI: 10.7554/eLife.49500
AI Summary: OriGene contributed to this study by providing a custom-generated TrueORFGold clone encoding a C-terminal His/DDK tagged IFI16, which was used to investigate IFI16's role in epigenetic silencing of herpesvirus genomes.
Snippet: sing plasmid IFI16-FL (pcDNA3-FLAG) was a gift from Cheryl Arrowsmith (Addgene plasmid 35064) (Liao et al., 2011). TrueORFGold clone encoding a C-terminal His/DDK tagged IFI16 was custom generated by Origene. The LANA-1 overexpressing plasmid pCI-neo full-length LANA-1 was described previously (Paudel et al., 2012). Cells were transfected using the TransIT-X2 Transfection Reagent (Mirus #MIR 6000)
FigureFigureFigure
Up-regulation of Interferon-inducible protein 16 contributes to psoriasis by modulating chemokine production in keratinocytes
Cao, Shao, Li et al
Sci Rep (2016) 6, 25381 DOI: 10.1038/srep25381
Cited by 43
AI Summary: In this study, the OriGene plasmid encoding IFI16 (RC202193) was used to overexpress IFI16 in human primary keratinocytes, helping to investigate its role in psoriasis-related signaling and chemokine production.
Snippet: Human primary keratinocytes were transfected with siRNAs targeting IFI16 and TBK1 (Q000003428, Q000029110;Ribobio, Guangzhou, China) or with plasmid encoding IFI16 (RC202193;OriGene, Rockville, MD, USA) according to the manufacturer's instructions using ... Cite SaveSaving
FigureFigureFigure
Showing 1-3 of 3 papers.
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