Tnfaip3 (NM_009397) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR210582
Tnfaip3 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1
$1,064.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tnfaip3 |
| Synonyms | A20; Tnfip3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009397 |
| ORF Size | 2325 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009397.1, NM_009397.2, NM_009397.3, NP_033423.3 |
| RefSeq Size | 4437 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 21929 |
| UniProt ID | Q60769 |
| Cytogenetics | 10 8.08 cM |
| MW | 87.7 kDa |
| Summary | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009397" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG210582 | Tnfaip3 (tGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3) | 10 ug |
$1,264.00
|
|
| MR210582L3 | Lenti ORF clone of Tnfaip3 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1 | 10 ug |
$1,364.00
|
|
| MR210582L4 | Lenti ORF clone of Tnfaip3 (mGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3), transcript variant 1 | 10 ug |
$1,364.00
|
|
Citations
Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific manner
Ding, Hu, Nguyen et al
Nat Commun (2025) 16 (1), 8839 DOI: 10.1038/s41467-025-63941-x
Ding, Hu, Nguyen et al
Nat Commun (2025) 16 (1), 8839 DOI: 10.1038/s41467-025-63941-x
Cited by 3
AI Summary: OriGene’s mouse A20 overexpression plasmid (OriGene, #MR210582) was used in this study to investigate the role of A20 in regulating neutrophil activation and survival following prenatal Zika virus exposure.
Snippet: h 1% penicillin/streptomycin and 10% BSA). For transfection, a mixture was prepared containing 6 μL of Escort III transfection reagent (Sigma, #L3037), 2 μg of mouse A20 overexpression plasmid (Origene, #MR210582), and 91.5 μL of Opti-MEM. The mixture was incubated at room temperature for 20 min in a biosafety hood before being added dropwise to the wells. Plates were gently swirled to
STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3/A20
Antonia, Karelehto, Toriguchi et al
J Cell Mol Med (2022) 26 (16), 4591-4601 DOI: 10.1111/jcmm.17489
Antonia, Karelehto, Toriguchi et al
J Cell Mol Med (2022) 26 (16), 4591-4601 DOI: 10.1111/jcmm.17489
Cited by 37
AI Summary: OriGene contributed to this study by providing the Tnfaip3/A20 overexpression plasmid (MR210582L4), which was used to enforce A20 expression in STAT3 knockout MEFs and assess its role in regulating inflammatory chemokine production.
Snippet: … Transfer plasmid containing the A20 insert was obtained from Origene (MR210582L4, Tnfaip3 NM_009397, OriGene Technologies, Inc., MD, USA). …Transfer plasmid containing the A20 insert was obtained from Origene (MR210582L4, Tnfaip3 NM_009397, OriGene Technologies, Inc., MD, USA)
Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages
Liu, Lv, Liu et al
Immunity (2019) 51 (3), 522-534.e7 DOI: 10.1016/j.immuni.2019.06.014
Liu, Lv, Liu et al
Immunity (2019) 51 (3), 522-534.e7 DOI: 10.1016/j.immuni.2019.06.014
Cited by 87
AI Summary: In this study, OriGene’s mouse C/EBPβ plasmid (Cat# MR226779) and mouse A20 plasmid (Cat# MR204921) were used to overexpress these proteins in functional experiments, helping to demonstrate their roles in the regulation of lung fibrosis.
Targeting Degradation of the Transcription Factor C/EBPβ Reduces Lung Fibrosis by Restoring Activity of the Ubiquitin-Editing Enzyme A20 in Macrophages
Liu, Lv, Liu et al
Immunity (2019) 51 (3), 522-534.e7 DOI: 10.1016/j.immuni.2019.06.014
Liu, Lv, Liu et al
Immunity (2019) 51 (3), 522-534.e7 DOI: 10.1016/j.immuni.2019.06.014
Showing 1-4 of 4 papers.
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