IHC Antibodies (Neurology)

Immunohistochemical staining of paraffin-embedded human embryonic liver tissue using ALDH1L1 clone UMAB43 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min in pressure chamber/cooker. UM500039 was diluted 1:400 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the hepatocyte.

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PANK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GBA mouse monoclonal antibody. (UM800064; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC1 mouse monoclonal antibody. (UM500037, dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-SYP mouse monoclonal antibody. (UM500079; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDGFRB mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FXN mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SIL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PDSS2 mouse monoclonal antibody. (UM500108, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-XPF (UMAB20) mouse monoclonal antibody. (UM500020, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHCHD10 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ANXA11 plasmid (RC212191, hANXA11), mouse ANXA11 plasmid (MR225524, mANXA11) using anti-ANXA11 antibody TA500950 (1:5000@1mg/ml). Figure B, Western blot analysis of the same human ANXA11 and mouse ANXA11 samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EGR2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SYNE1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ITM2B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NEFL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PMP22 mouse monoclonal antibody.This figure shows negative staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human HSPB8 plasmid (RC201040, hHSPB8) using anti-HSPB8 antibody TA501210 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GARS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M. Positive lysates LY401311 (100ug) and LC401311 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KARS mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-GFAP mouse monoclonal antibody. (UM500094; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

Immunohistochemical staining of paraffin-embedded normal breast using S100B antibody TA807235 clone OTI3G6 mouse monoclonal antibody. Protocol used HIER TEE pH9.0 (cat# B21-100)and anti- S100B at 1:5000 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NSE (ENO2) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800177) (1:15000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNCA mouse monoclonal antibody. (TA506533) (1:150). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC8 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GRN mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EIF2B3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CTDSP2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PIK3R5 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDXK mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-APOE mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human LMNA plasmid (RC204970, hLMNA), mouse LMNA plasmid (MR209880, mLMNA) using anti-LMNA antibody TA803494 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DNAJB2 (Cat# RC206769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB2(Cat# TA502760). Positive lysates LY422070 (100ug) and LC422070 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-EIF2AK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800132) (1:100)

Immunohistochemical staining of paraffin-embedded prostate cancer using anti-HTT C/N TA591054 clone OTI2E10 rabbit monclonal antibody. HIER Accel buffer pH8.7 (B22-125) at 110C for 10 min, anti-HTT diluted to 1:100. Detection was done with Polink1 Rabbit C/N D13-18 with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LYZ mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-OPTN mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.