EV Intactness

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO, Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Anti-HSPA1A mouse monoclonal antibody (TA500772) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA1A (RC200270).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SDCBP (Cat# RC215390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDCBP (Cat# TA504796). Positive lysates LY417182 (100ug) and LC417182 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDCD6IP (Cat# RC203735, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD6IP(Cat# TA507312). Positive lysates LY415634 (100ug) and LC415634 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein HSP90AB1 from HELA lysates (0.2 mg/mL) using HSP90AB1 Mouse Monoclonal Antibody #TA500494. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANXA10 (Cat# RC200268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA10 (Cat# TA500882). Positive lysates LY402103 (100ug) and LC402103 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 9 different cell lines by using Rabbit Polyclonal anti-GAPDH antibody at 1:2000 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Western blot analysis of TSG101 in K562, rat Brain, C6, 3T3, Hela lysates using TSG101 antibody.