GAPDH Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | Simple Western, WB |
|---|---|
| Recommended Dilution | WB 1:2000, IHC 1:150 Simple Western 1:50-1:100 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 220 - 290 of Human GAPDH. |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Purification | Purified from the immunized serum by affinity chromatography (Protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | glyceraldehyde-3-phosphate dehydrogenase |
| Database Link | |
| Background | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2012 |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other antibodies (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA890003S | Rabbit polyclonal anti-GAPDH antibody, Loading control | 30 ul |
$188.00
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Citations
A Novel Microfluidic System for Immunomagnetic Isolation of Pancreatic Cancer CTCs: Plectin‑1 and EpCAM as Potential Tumor Markers
Baranowska, Łuszczuk, Kołodziejek et al
ACS Omega (2026) 11 (17), 25266-25276 DOI: 10.1021/acsomega.5c12181
Baranowska, Łuszczuk, Kołodziejek et al
ACS Omega (2026) 11 (17), 25266-25276 DOI: 10.1021/acsomega.5c12181
AI Summary: OriGene’s GAPDH antibody (catalog TA890003) was used as a loading control in western blot analyses to validate protein expression in the study developing a microfluidic system for isolating pancreatic cancer circulating tumor cells.
Snippet: Equal amounts of protein lysates (20 μg) were separated by SDS-PAGE, transferred to PVDF membranes, and probed with antibodies against Plectin-1 (catalog AB32528, Abcam;dilution 1:2500) and GAPDH (catalog TA890003, OriGene, Austin, TX, USA;dilution 1:2500). Protein bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad)
KSHV and HPV modulate epithelial-to-mesenchymal transition in oral epithelial cells
Li, Hopcraft, Lange et al
mBio (2025)
Li, Hopcraft, Lange et al
mBio (2025)
Cited by 3
Snippet: K10 (Proteintech, 18343-1-AP),glyceraldehyde phosphate dehydrogenase (GAPDH) (OriGene, TA890003S), E-cadherin (Invitrogen, 33-4000), vimentin (Proteintech, 10366-1-AP), ZO-1 (Cell Signaling Technology, 8193T), beta-catenin (Cell Signaling Technology, 8480T),
ATP-induced fibrogenic pathway in circulating cells obtained by idiopathic pulmonary fibrotic (IPF) patients is not blocked by nintedanib and pirfenidone
Colarusso, Falanga, Di Caprio et al
Biomed Pharmacother (2024) 176, 116896 DOI: 10.1016/j.biopha.2024.116896
Colarusso, Falanga, Di Caprio et al
Biomed Pharmacother (2024) 176, 116896 DOI: 10.1016/j.biopha.2024.116896
Cited by 6
AI Summary: OriGene’s GAPDH antibody (#TA890003) was used as a loading control in western blot experiments to ensure equal protein loading in the study.
Snippet: GAPDH (35 kDa diluted 1:2000 in a PBS 1× solution containing 5 %BSA;# TA890003;OriGene Technologies, Rockville, MD, USA#) was used as loading control. The signals were detected with anti-rabbit horseradish peroxidase-conjugated antibody (diluted 1:3000 in a PBS 1× solution containing 5 %BSA;# GtxRb-003-DHRPX;ImmunoReagents Inc.), detected by using
Sex Differences in Sphingosine-1-Phosphate Levels Are Dependent on Ceramide Synthase 1 and Ceramidase in Lung Physiology and Tumor Conditions
Terlizzi, Colarusso, Ferraro et al
Int J Mol Sci (2023) 24 (13) DOI: 10.3390/ijms241310841
Terlizzi, Colarusso, Ferraro et al
Int J Mol Sci (2023) 24 (13) DOI: 10.3390/ijms241310841
Cited by 4
AI Summary: OriGene products GAPDH (#TA890003) and HSC-70 (#TA332519) antibodies were used as loading controls in protein expression analyses to ensure accurate quantification of target proteins in the study.
Snippet: sor form: 55 kDa;N-acylsphingosine amidohydrolase 1, ASAH1;Elabscience;Houston, TX, USA;#E-AB-10959) was evaluated in ADK and SCC tissues. Glyceraldehyde-3-phosphate dehydrogenase (37 kDa;GAPDH;OriGene Technologies, Rockville, MD, USA;#TA890003) or heat shock cognate protein 70 (70 kDa;HSC-70;#TA332519) were used as the loading control. Data were
Exposure to nanoplastics induces mitochondrial impairment and cytomembrane destruction in Leydig cells
Sun, Wen, Zhang et al
Ecotoxicol Environ Saf (2023) 255, 114796 DOI: 10.1016/j.ecoenv.2023.114796
Sun, Wen, Zhang et al
Ecotoxicol Environ Saf (2023) 255, 114796 DOI: 10.1016/j.ecoenv.2023.114796
Cited by 115
AI Summary: OriGene’s GAPDH antibody (Cat. No. TA890003) was used as a loading control in western blot experiments to assess protein expression changes in Leydig cells exposed to nanoplastics.
Snippet: …AF0120), and GAPDH (1:5000, OriGene Technologies, China;Cat. No. TA890003) at 4 °C overnight
S1P-Induced TNF-α and IL-6 Release from PBMCs Exacerbates Lung Cancer-Associated Inflammation
Terlizzi, Colarusso, Somma et al
Cells (2022) 11 (16) DOI: 10.3390/cells11162524
Terlizzi, Colarusso, Somma et al
Cells (2022) 11 (16) DOI: 10.3390/cells11162524
Cited by 30
AI Summary: OriGene's Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; #TA890003) antibody was used as a loading control in Western blot analyses to ensure accurate quantification of protein expression in PBMCs treated with S1P in this study.
Snippet: , USA) expression were evaluated after one hour treatment of PBMCs with S1P (10 nM). Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH;diluted 1:2000 in a PBS 1× solution containing 5%BSA;#TA890003;OriGene Technologies, Rockville, MD, USA) was used as loading control. Data were analysed by means of ImageJ software 1.53a http://imagej.nih.gov/ij (NIH, Bethesda, MD, USA). 2.5. Statistical Analysi
Showing 1-6 of 19 papers.
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