TissueScan, Liver Cancer cDNA Array I

Snippet: care of patients and detecting HCC-NC at early stages. 2. Materials and Methods2.1. Study of cDNA Array of HCC Patients Commercially available TissueScan Liver Cancer cDNA Array I (Cat # LVRT101) of Origene (Rockville, MD, USA) was utilized using the manufacturer’s protocol to assess HADHA transcript levels. The array plate had cDNAs from eight normal and twenty-six liver cancer tissues at diff

Snippet: R was used to analyze the relative expression of PSMD1 in patient tissues and confirm the knockdown efficiency of siRNA in transfected cells. The Tissuescan Liver Cancer cDNA Array was purchased from OriGene (LVRT101, Rockville, MD, USA). The mRNA levels of PSMD1 and β2-microglobulin (β2M)32 were measured using real-time PCR using a KAPA SYBR FAST Universal qPCR kit (KK4301, Kapa Biosystems, Inc

Snippet: instructions, as previously reported [54]. 4.14. cDNA Array TissueScan cDNA Array (Liver Cancer cDNA Array I, Origene) was used to quantify BCL-xL and MCL-1 levels in tumor and normal tissues. Tissue cDNAs of each array

Snippet: http://cancergenome.nih.gov/) and were re-analyzed using Oncomine (https://www.oncomine.org). qPCR was performed on the TissueScan Cancer cDNA Array obtained from Origene Technologies 204345). cDNA was added to 25 ng/μl (cell line cDNAs)