TissueScan, Ovarian Cancer cDNA Array I

Snippet: The receptor activator of nuclear factor kappa B (RANK) is becoming recognized as a master regulator of tumorigenesis, yet its role in gynecological cancers remains mostly unexplored. We investigated whether there is a gradation of RANK protein and mRNA expression in epithelial ovarian cancer (EOC) according to malignancy and tumor staging. Immunohistochemical expression of RANK was examined in a ...

Snippet: designed to be specific for CD44 variants (SUPPLEMENTARY METHODS). TissueScan, Ovarian Cancer cDNA Array I (Cat.# HORT101) was purchased from Origene (Rockville, MD). Gene Editing with CRISPR/Cas9. CRISPR/Cas9 plasmids were designed and

Snippet: Sample collection and preparation Tissue cDNA Array plates were purchased from Origene (Rockville, MD, USA), consisting of three cancer subtypes: lung, ovarian and breast, with five individual patient samples per cancer type, including one normal control View as HTML

Snippet: Cells were incubated at 37°C for 48 hours, and MCA formation was confirmed under the light microscope. Quantitative Polymerase Chain Reaction of cDNA Arrays. Ovarian cancer TissueScan cDNA arrays (HORT-01) were obtained from OriGene.

Snippet: Human samples Frozen or paraffin-embedded ovarian tumour samples were obtained from the Department of Pathology, Taipei Veterans General Hospital, under the approved IRB protocol. Informed written consent was obtained from all patients who participated in the study. Tumour samples were collected during debulking surgery and patient’s identity of pathological specimens remained anonymous. Detailed ...