TissueScan, Human Normal cDNA Array

Snippet: (Qiagen, Venlo, The Netherlands). cDNA was generated using SuperScript III Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA, #18090050). A cDNA panel of 48 normal human tissues was purchased from OriGene (Rockville, MD, USA) (HMRT104). RNA concentrations were measured using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific). RT-qPCR was performed with TaqMan Fast Advanced Master M

Snippet: Quantitative PCR (qPCR) was performed on cDNA panels of 48 healthy human tissues (OriGene Cat# HMRT104, Technologies, Rockville, MD) and TNBC cell lines using MX3000 [RRID:SCR_020526;Taqman probes ROPN1: Hs00250195_m1;ROPN1B: Hs00250195_m1;CTAG1B (NY-ESO1): Hs00265824_m1;GAPDH: Hs02758991_g1;NBL1: Hs01063631_m1;UBE2O: Hs00222904_m1;FAAH: Hs01038664_m1]. …

Snippet: The human normal tissue cDNA array (Cat. #HMRT104, OriGene, Rockville, MD, USA) was removed from the −20 ℃ refrigerator, centrifuged once for a short time, and equilibrated to

Snippet: meters of the Applied Biosystems Step One Plus real-time PCR system (for 96-well format). AR45 differential expression in normal human tissues was detected by RT-qPCR using the TissueScan cDNA Array (Origene, HMRT104). Oligonucleotides used in the RT-qPCR experiments are listed in table S2. AR45 siRNA screens and AR45 KD experiments A panel of 11 siRNAs targeting all possible regions specific for

Snippet: according to the manufacturer's instructions. Human normal tissue cDNA array was purchased from OriGene Technologies (Rockville, MD). Each PCR procedure included a non-template negative control reaction. The level of 18s;- according to the manufacturer's instructions. Human normal tissue cDNA array was purchased from OriGene Technologies (Rockville, MD). Each PCR procedure included a non-templa...

Snippet: cells and human MAFLD. Real-time quantitative polymerase chain reaction was used to assess TLL1 mRNA expression in a selection of 18 human tissues using TissueScan Human Normal cDNA Array (Origene, Rockville, MD) Cached