TissueScan, Brain Cancer cDNA Array I

Snippet: ssion data in the Human Protein Atlas (https://www.proteinatlas.org) as reported in GenTree (gentree.ioz.ac.cn), a database comparing species-specific gene assembly (29), and (ii) qRT-PCR of cDNAs in OriGene Tissue Scan Arrays prepared of normal human peripheral tissues and human brain. Recruitment of Human Volunteers for Blood Donation. Blood was collected from normal and burn patients after inf

Snippet: We performed total mRNA extraction, reverse transcription and quantitative RT-PCR according to the manufacturer’s protocol. Normal brain cDNA was purchased from Origene Technologies (Rockville, MD, USA). The reverse transcripts were amplified and quantified using an Illumina ECO Real-Time PCR system

Snippet: Top of page Materials and methods. Molecular approach of Ptchd1 function. The expression profile of Ptchd1 was assessed using the TissueScan quantitative PCR-ready cDNA Arrays (Origene, Rockville, MD, USA;Supplementary Methods). ... All 3 versions Cite SaveSaving

Snippet: TissueScan Brain Cancer Tissue cDNA array I, containing 46 malignant (covering four stages) and 2 tumor-adjacent normal tissue cDNAs, were obtained from Origene Technologies, (Rockville, MD, USA)