TissueScan, Human Brain cDNA Array

Snippet: /13/03/00/15). All the cell lines were anonymized. DNA and RNA Analysis. For expression analysis, human multiple tissue cDNA panel (MTC Clontech, 636742) and human normal brain tissue qPCR array (OriGene Technologies, HBRT101) were used. Prior to their use, these samples were deidentified by manufacturers. Detailed methods are described in SI Appendix, Materials and Methods. Supplementary Ma

Snippet: …Human cDNA panels were obtained from OriGene: TissueScan, Human Brain cDNA Array (#HBRT101) and Human Normal cDNA Array (#HMRT304). … In Silico modelling of FIBCD1 Docking solutions In silico;Human cDNA panels were obtained from OriGene: TissueScan, Human Brain cDNA Array (#HBRT101) and Human Normal cDNA Array (#HMRT304). …

Snippet: …Methods Cell;- Clinical samples across all stages of the disease were acquired from Origene (USA) as Tissue scan colon cancer cDNA array V (Cat No. HCRT105). …

Snippet: The different regions of the normal human brain cDNA obtained from OriGene Biotechnology was directly subjected to RT-qPCR using hSVCT2 or hCLSTN3 specific primers (Table 1). β-Actin was used as a reference target to normalize relative expression values Save;- The different regions of the normal human brain cDNA obtained from OriGene Biotechnology was directly subjected to RT-qPCR using hSVC...

Snippet: DNA and RNA analysis For expression analysis human multiple tissue cDNA panel (MTC Clontech, 636742) and human normal brain tissue qPCR array (OriGene Technologies, HBRT101) were used. 1-3 ng of cDNA was used for real-time PCR assay

Snippet: ech). _FAM50A_ was expressed in all eight fetal tissues assessed, including brain (Supplementary Fig. 3a). Next, we evaluated _FAM50A_ expression in fetal brain using a Rapid Scan Human Brain Panel (Origene). _FAM50A_ was detectable in the fetal cerebellum and hypothalamus (Supplementary Fig. 3b). However, we observed low expression of _FAM50A_ in the temporal lobe and were unable to detect expr;- Intellectual disability (ID) is a heterogeneous clinical entity and includes an excess of males who harbor variants on the X-chromosome (XLID). We report rare FAM50A missense variants in the original Armfield XLID syndrome family localized in Xq28 and four additional;ght fetal tissues assessed, including brain (Supplementary Fig. 3a [/articles/s41467-020-17452-6#MOESM1]). Next, we evaluated _FAM50A_ expression in fetal brain using a Rapid Scan Human Brain Panel (Origene). _FAM50A_ was detectable in the fetal cerebellum and hypothalamus (Supplementary Fig. 3b [/articles/s41467-020-17452-6#MOESM1]). However, we observed low expression of _FAM50A_ in the tempor;lontech). FAM50A was expressed in all eight fetal tissues assessed, including brain (Supplementary Fig. 3a). Next, we evaluated FAM50A expression in fetal brain using a Rapid Scan Human Brain Panel (Origene). FAM50A was detectable in the fetal cerebellum and hypothalamus (Supplementary Fig. 3b). However, we observed low expression of FAM50A in the temporal lobe and were unable to detect expressi